Development and Integration of Bioluminescent CT

生物发光CT的开发和集成

• 批准号: 7368492
• 负责人: Ge Wang
• 金额: $ 12.96万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-14 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7368492
• 关键词: X ray; bioimaging /biomedical imaging; bioluminescence; biomedical equipment development; computed axial tomography; gene expression; imaging /visualization /scanning; laboratory mouse; lung

DESCRIPTION (provided by applicant): Small animals, particularly genetically engineered mice, are of increasing importance to understand the biologic basis for pathologic disease manifestations. Major efforts are being made to link the genome to the phenotypic expression in both form and function ("Physiome"). Small animal imaging offers the opportunity to evaluate pathologic progression in a much-compressed time frame and with a much-improved resolution. In this R21/R33 proposal, we plan to develop and integrate the first bioluminescent CT (BLCT) device for imaging gene expression and a state-of-the-art micro-CT scanner for studies of the lung in the living mouse model. Our long term goal is to advance molecular and micro imaging technology, and make the proposed integrated system an in vivo tool in biomedical applications, especially for small animal tomography. The specific aims of the R21 Phase are to (1) develop a data acquisition module for bioluminescent imaging of the mouse lung; (2) establish the forward model for bioluminescence based on the optical properties of the mouse as derived from its CT volume; (3) demonstrate the feasibility of bioluminescent tomography in numerical simulation and phantom experiments. The specific aims of the R33 Phase are to (1) prototype a bioluminescent CT device with I0 CCD cameras, and develop an iterative algorithm for a spatial resolution on the order of 1 ram; (2) build a multi-resolution micro-CT scanner with dual imaging, and develop analytic algorithms for high quality reconstructions, including the in vivo mode with about 20 micron spatial resolution and 20 second temporal resolution and a maximal spatial resolution on the order of 5 microns; (3) integrate the two cutting-edge CT modalities (optical and X-ray), and demonstrate the feasibility and utility of the integrated system in small animal studies. Upon completion of the R21/33 project, a unique micro-tomography system will have been developed with molecular, multi-resolution, multi-energy, dynamic and bioluminescent imaging capabilities. We will demonstrate, through example projects, the utility of the system in generating critical physiological and pathological information of the lung.

描述（由申请人提供）： 小动物，特别是基因工程小鼠，对于了解病理疾病表现的生物学基础变得越来越重要。人们正在努力将基因组与形式和功能上的表型表达联系起来（“Physiome”）。小动物成像提供了在大大压缩的时间范围内以大大提高的分辨率评估病理进展的机会。在这个 R21/R33 提案中，我们计划开发和集成第一个用于基因表达成像的生物发光 CT (BLCT) 设备和用于研究活体小鼠模型肺部的最先进的微型 CT 扫描仪。我们的长期目标是推进分子和显微成像技术，并使所提出​​的集成系统成为生物医学应用中的体内工具，特别是小动物断层扫描。 R21阶段的具体目标是（1）开发用于小鼠肺部生物发光成像的数据采集模块； (2) 根据小鼠 CT 体积得出的光学特性，建立生物发光正演模型； (3)论证了生物发光断层扫描在数值模拟和体模实验中的可行性。 R33阶段的具体目标是（1）使用I0 CCD相机制作生物发光CT设备原型，并开发空间分辨率达到1 ram量级的迭代算法； （2）构建具有双成像的多分辨率微型CT扫描仪，并开发高质量重建的分析算法，包括约20微米空间分辨率和20秒时间分辨率的体内模式以及约20秒的最大空间分辨率5微米； （3）整合两种尖端CT模式（光学和X射线），并证明集成系统在小动物研究中的可行性和实用性。 R21/33项目完成后，将开发出一种独特的显微断层扫描系统，具有分子、多分辨率、多能量、动态和生物发光成像能力。我们将通过示例项目演示该系统在生成肺部关键生理和病理信息方面的实用性。