Bandwidth-Selective, APD-Based Flow Cytometer

基于 APD 的带宽选择性流式细胞仪

• 批准号: 6932100
• 负责人: JAMES F CHRISTIAN
• 金额: $ 44.55万
• 依托单位: RADIATION MONITORING DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932100
• 关键词: computer program /software; computer system design /evaluation; computer system hardware; flow cytometry; lasers; optics; photonics; technology /technique development

DESCRIPTION (provided by applicant): An ultra-compact, high-performance, multi-laser, many-parameter, bandwidth-selective flow cytometer is the expected result of the research and development proposed here. The cellular analysis typically carried out by a device of this type, through the use of lasers and photon detectors, will be moved to a whole new level of capability with the research detailed in this proposal. Specifically, revolutionary developments in the basic components of the cytometer: the focusing optics, the laser-interrogation system, and the photon-detection systems are proposed based upon several exciting advancements in these critical areas. Proposed research in optics will develop a sophisticated laser-focusing aperture that can be used with new powerful and compact solid-state lasers in order to increase the number of lasers used in a many-parameter, flow-cytometer system. State-of-the-art research in miniature, solid-state photon detectors will result in highly sensitive, silicon photodetectors, or avalanche photodiodes, that can be adapted for use in flow cytometers. These photon detectors will be used to replace conventional, cumbersome photomultiplier tubes. The current widespread use of flow cytometers as a method of diagnosing disease, especially cancer, through cell population analysis will benefit highly from the increase in sensitivity and capability provided by development of the high-performance device proposed here. Commercially, flow cytometers have wide market acceptance for use as core diagnostic devices in pathology and immunology departments in many hospitals across the United States. Since the market is established for these devices, our high-performance, extremely compact device should find broad acceptance and provide immediate technological improvement in the diagnosis and science carried out with flow cytometers.

描述（由申请人提供）：超紧凑，高性能，多激光，多参数，带宽选择性流式细胞仪是此处提出的研究和开发的预期结果。通过使用激光和光子探测器，通过这种类型的设备进行的细胞分析将通过本提案中的研究详细介绍，将移动到全新的能力水平。 具体而言，基于这些关键领域的几个令人兴奋的进步，提出了细胞仪基本组成部分中的革命性发展：聚焦光学元件，激光介入系统和光子检测系统。拟议的光学研究将开发出复杂的激光孔，可与新的功能强大且紧凑的固态激光器一起使用，以增加在多参数，流程 - 流程机系统中使用的激光数。微型，固态光子检测器的最先进的研究将导致高度敏感的硅光电探测器或雪崩光电二极管，可用于流式细胞仪。这些光子探测器将用于替代常规的，繁琐的光电倍增管。 流式细胞仪当前通过细胞种群分析将流式细胞仪作为诊断疾病的一种方法，将受益于在此处提出的高性能装置的开发所提供的灵敏度和能力的提高。在商业上，流式细胞仪在美国许多医院的病理学和免疫学部门的核心诊断设备中都有广泛的市场认可。由于为这些设备建立了市场，因此我们的高性能，非常紧凑的设备应找到广泛的接受度，并可以立即通过流式细胞仪进行诊断和科学方面的技术改进。