Hybrid Transform Mass Spectrometer for Proteomics

用于蛋白质组学的混合变换质谱仪

基本信息

批准号：
6803692
负责人：
PATRICK A LIMBACH
金额：
$ 91.9万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-25 至 2006-04-24
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6803692
关键词：
biomedical equipment purchase biotechnology mass spectrometry proteomics

项目摘要

DESCRIPTION (provided by applicant): This proposal requests support for the acquisition of an ion trap-Fourier transform ion cyclotron resonance hybrid mass spectrometer. This new instrumentation is intended for the identification and quantitation of proteins from protein complexes, whole proteomes and single cells. This instrument is arguably at the forefront of mass spectrometry instrumentation, and represents a new platform for undertaking sophisticated proteomics-related studies. Proposed use of this instrumentation will support and enhance existing NIH-funded projects and provide avenues of research support that can lead to new funding for future projects. Four NIH-supported users of this equipment will form the core research team and the value added to their research activities by this instrumentation is discussed. All of these individuals currently interact with the University of Cincinnati Mass Spectrometry (UC MS) Facility, and the identified projects are untenable or pain-staking using the equipment already in place in the UC MS Facility. Their research interests, in some form or another, cover a wide spectrum of proteomics-related investigations. Examples include the effects of environmental agents such as estrogens, metals and PAH's on proteomes; examining the underlying biochemistry associated with microorganisms as they relate to biofilm production; and understanding the lamellar body proteome. All of the investigators participating in this instrument request have vigorous and well-funded research programs. In addition, a number of other investigator's NIH-funded research would benefit from this instrument acquisition. The PI, who has been active in the field of mass spectrometry for nearly 15 years, will be responsible for the managerial oversight of the requested instrumentation. He will report to an internal advisory board composed of scientists from various UC campuses and affiliates. The internal advisory board will establish scheduling priorities, work with the PI and accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility was renovated in 2001 and is equipped with the ancillary instrumentation required for proteomics. The instrumentation will be operated and maintained by two Ph.D.-level facility staff scientists, with one of these scientists having prior experience in both FTMS and proteomics being designated as the primary operator. These staff scientists are supported by B.S.-level staff technicians to ensure that the services are provided in a timely manner.

描述（由申请人提供）：该提案请求支持购买离子阱-傅里叶变换离子回旋共振混合质谱仪。这种新仪器旨在对蛋白质复合物、整个蛋白质组和单细胞中的蛋白质进行鉴定和定量。该仪器可以说处于质谱仪器的最前沿，代表了进行复杂的蛋白质组学相关研究的新平台。该仪器的拟议使用将支持和加强现有的 NIH 资助项目，并提供研究支持途径，从而为未来项目带来新的资金。该设备的四名 NIH 支持用户将组成核心研究团队，并讨论该仪器为其研究活动带来的附加值。所有这些人目前都与辛辛那提大学质谱 (UC MS) 设施进行互动，而使用 UC MS 设施中已有的设备来确定的项目是站不住脚的或费力的。他们的研究兴趣以某种形式涵盖了广泛的蛋白质组学相关研究。例如雌激素、金属和多环芳烃等环境因素对蛋白质组的影响；检查与微生物相关的潜在生物化学，因为它们与生物膜的产生有关；并了解层状体蛋白质组。所有参与该仪器请求的研究人员都有积极且资金充足的研究计划。此外，美国国立卫生研究院 (NIH) 资助的许多其他研究人员的研究也将受益于该仪器的采购。该 PI 在质谱领域活跃了近 15 年，将负责所需仪器的管理监督。他将向由来自加州大学各个校区和附属机构的科学家组成的内部顾问委员会报告。内部顾问委员会将确定调度优先级，与 PI 和会计人员合作确定成本回收率，并将作为预测所需设备的未来使用情况的资源。该设备将安置在辛辛那提大学质谱设施中。该设施占地 3,500 平方英尺，于 2001 年进行了翻修，配备了蛋白质组学所需的辅助仪器。该仪器将由两名博士级科学家负责操作和维护，其中一名在 FTMS 和蛋白质组学方面拥有丰富经验的科学家被指定为主要操作员。这些科学家得到了学士学位级别的技术人员的支持，以确保及时提供服务。