Acquisition of a High Resolution Mass Spectrometer for the University of Cincinna

为辛辛那大学购置高分辨率质谱仪

• 批准号: 8734519
• 负责人: PATRICK A LIMBACH
• 金额: $ 96.35万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8734519
• 关键词: Accounting; Antibiotic Resistance; Atherosclerosis; Cholesterol; Communicable Diseases; Diabetes Mellitus; Disease; Doctor of Philosophy; Equipment; Fourier Transform; Funding; Future; Goals; Health; Housing; Human Resources; Liquid Chromatography; Lysophospholipids; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Metabolic; Metabolic Pathway; Obesity; Recovery; Reporting; Request for Proposals; Research; Research Personnel; Resolution; Resources; Schedule; Scientist; System; United States National Institutes of Health; Universities; Work; cost; flu; instrument; instrumentation; mass spectrometer; metabolomics; research study

DESCRIPTION (provided by applicant): This proposal requests funds to acquire a high resolution Fourier transform mass spectrometer and associated liquid chromatography system to support the NIH-funded research of investigators at the University of Cincinnati. This new instrumentation is intended to support on- going NIH-funded research into health-related issues such as antibiotic resistance, atherosclerosis, cancer, diabetes, infectious diseases and obesity. Specifically, the following types of analyses will be enabled by this acquisition: a) Metabolic flu measurements to identify key metabolic pathways in cardiometabolic diseases and various cancers; b) Metabolite identification to support identification of metabolic pathways revealed during metabolic flux measurements; and c) metabolomic characterization of lysophospholipid and cholesterol metabolic pathways. A key group of NIH-supported investigators at the University of Cincinnati will directly benefit from these new capabilities. Moreover, these new capabilities will stimulate these researchers and other biomedical investigators on campus to examine additional experiments that will benefit from this instrument. The NIH-funded researchers participating in this request require dedicated LC-MS/MS or high-resolution ESI/APCI-MS/MS capabilities to accomplish goals associated with their funded projects. The PI, who has been active in the field of mass spectrometry for over 20 years, will be responsible for the managerial oversight of the requested instrumentation. He will report to an external advisory board composed of scientists from within the university. The advisory board will establish scheduling priorities, work with the PI and accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility is equipped with the ancillary instrumentation required for biomolecule characterization. The instrumentation will be operated and maintained by two Ph.D.-level facility staff scientists, with one of these scientists being designated as the primary operator.

描述（由申请人提供）：该提案请求资金购买高分辨率傅里叶变换质谱仪和相关的液相色谱系统，以支持 NIH 资助的辛辛那提大学研究人员的研究。这种新仪器旨在支持美国国立卫生研究院 (NIH) 资助的针对抗生素耐药性、动脉粥样硬化、癌症、糖尿病、传染病和肥胖等健康相关问题的持续研究。具体来说，此次收购将实现以下类型的分析： a) 代谢流感测量，以确定心脏代谢疾病和各种癌症的关键代谢途径； b) 代谢物鉴定，以支持代谢通量测量期间揭示的代谢途径的鉴定； c) 溶血磷脂和胆固醇代谢途径的代谢组学特征。辛辛那提大学由美国国立卫生研究院支持的研究人员组成的一个关键小组将直接受益于这些新功能。此外，这些新功能将刺激这些研究人员和校园内的其他生物医学研究人员检查将从该仪器中受益的其他实验。参与此请求的 NIH 资助的研究人员需要专用的 LC-MS/MS 或高分辨率 ESI/APCI-MS/MS 功能来实现与其资助项目相关的目标。 PI 在质谱领域活跃了 20 多年，他将负责对所需仪器的管理监督。他将向由大学内部科学家组成的外部顾问委员会报告。咨询委员会将确定调度优先级，与 PI 和会计人员合作确定成本回收率，并将作为预测所需设备的未来使用情况的资源。该设备将安置在辛辛那提大学质谱设施中。这个 3,500 平方英尺的设施配备了生物分子表征所需的辅助仪器。该仪器将由两名博士级设施科学家操作和维护，其中一名科学家被指定为主要操作员。