Acquisition of an LC Mass Spectrometer for Nucleic Acids Research

购买用于核酸研究的 LC 质谱仪

• 批准号: 7795317
• 负责人: PATRICK A LIMBACH
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795317
• 关键词: Accounting; Address; Antibiotic Resistance; Antineoplastic Agents; Base Sequence; Detection; Digestion; Doctor of Philosophy; Ensure; Equipment; Funding; Future; Goals; High Pressure Liquid Chromatography; Housing; Human Resources; Institution; Maps; Mass Spectrum Analysis; Measurement; Molecular Weight; Nucleic Acids; Nucleic acid sequencing; Nucleosides; Oligonucleotides; Peptides; Pseudouridine; Recovery; Request for Proposals; Research; Research Personnel; Resources; Scanning; Schedule; Scientist; Services; Side; System; Transfer RNA; Universities; Work; cost; crosslink; drug development; instrument; instrumentation; liquid chromatography mass spectrometry; mass spectrometer; nanobiotechnology; programs; public health relevance; square foot

DESCRIPTION (provided by applicant): This proposal requests funds to acquire an ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS) system intended for the identification and structural characterization of nucleic acids, including those containing modified nucleosides. The NIH-funded researchers participating in this request require LC-MS/MS or LC-IMS-MS/MS capabilities to accomplish goals associated with their funded projects. Specifically, the following types of analyses will be enabled by this acquisition: a) Accurate molecular weight measurements of intact nucleic acids; b) Accurate enzymatic mapping of isolated nucleic acids or mixtures of nucleic acids; c) Sequencing of oligonucleotides and enzymatic digestion mixtures by MS/MS; d) Identification of modified nucleosides and their placement within nucleic acid sequences; e) Sequence placement of pseudouridine, the most abundant modified nucleoside, using MRM scans; f) High sensitivity detection and identification of oligonucleotide synthetic side- and by-products; g) Detection of oligonucleotide:peptide cross-links; and h) de novo sequencing of modified RNAs, such as transfer RNAs (tRNAs). The PI, who has been active in the field of mass spectrometry for nearly 20 years, will be responsible for the managerial oversight of the requested instrumentation. He will coordinate oversight with an advisory board composed of scientists from various participating institutions. Together they will establish scheduling priorities, work with accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility was renovated in 2001 and is equipped with the ancillary instrumentation required for providing the proposed analytical services. The facility's co-directors are two Ph.D.-level staff scientists. One of these scientists will be designated as the primary operator of the new instrument, and it is anticipated that the facility will add a B.S.-level staff technician to ensure that existing and new analytical services are provided in a timely manner. PUBLIC HEALTH RELEVANCE: The UPLC-MS requested in this application will allow NIH-supported researchers access to modern instrumentation necessary for their research programs. These researchers are addressing a variety of health-related issues including: antibiotic resistance, cancer, drug development and nanobiotechnology.

描述（由申请人提供）：该提案要求资金购买超高性能液相色谱质谱（UPLC-MS）系统，用于核酸（包括含有修饰核苷的核酸）的鉴定和结构表征。参与此请求的 NIH 资助的研究人员需要 LC-MS/MS 或 LC-IMS-MS/MS 功能来实现与其资助项目相关的目标。具体来说，此次收购将实现以下类型的分析： a) 完整核酸的准确分子量测量； b) 分离的核酸或核酸混合物的准确酶图谱； c) 通过 MS/MS 对寡核苷酸和酶消化混合物进行测序； d) 修饰核苷的鉴定及其在核酸序列中的位置； e) 使用 MRM 扫描对最丰富的修饰核苷假尿苷进行序列定位； f) 寡核苷酸合成副产物的高灵敏度检测和鉴定； g) 寡核苷酸：肽交联的检测； h) 修饰RNA的从头测序，例如转移RNA (tRNA)。该 PI 在质谱领域活跃了近 20 年，将负责所需仪器的管理监督。他将与由来自各个参与机构的科学家组成的顾问委员会协调监督。他们将共同确定调度优先级，与会计人员合作确定成本回收率，并将作为预测所需设备的未来使用情况的资源。该设备将安置在辛辛那提大学质谱设施中。这个 3,500 平方英尺的设施于 2001 年进行了翻修，并配备了提供拟议分析服务所需的辅助仪器。该设施的联合主任是两名博士级科学家。其中一名科学家将被指定为新仪器的主要操作员，预计该设施将增加一名学士学位级别的技术人员，以确保及时提供现有和新的分析服务。 公共健康相关性：本申请中要求的 UPLC-MS 将使 NIH 支持的研究人员能够获得其研究项目所需的现代仪器。这些研究人员正在解决各种与健康相关的问题，包括：抗生素耐药性、癌症、药物开发和纳米生物技术。