Transgenic and Gene Targeting Core

转基因和基因靶向核心

基本信息

批准号：
7647669
负责人：
DIANA Mouhan SHIH
金额：
$ 19.8万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7647669
关键词：
3&apos Flanking Region 5&apos Flanking Region Agar Gel Electrophoresis Alleles Ampicillin Animal Model Animals Aorta Apolipoprotein E Area Ascaridil Atherosclerosis Back Backcrossings Bacterial Artificial Chromosomes Boxing Breeding Budgets Buffers C57BL/6 Mouse CD31 Antigens Candidate Disease Gene Cell Culture Techniques Cell Line Cells Chimeric Proteins Chloroform Clone Cells Cloning Color Communities Complementary DNA Congenic Mice Congenic Strain Consultations Core Facility Critiques DNA DNA Sequence DNA analysis DNA purification DUSP1 gene Data Data Set Databases Development Digestion Disease Documentation Dominant-Negative Mutation ES Cell Line Electroporation Elements Embryo Embryo Transfer Endothelial Cells Engineering Enhancers Ensure Enterochromaffin Cells Escherichia coli Ethanol Excision Exons Experimental Designs Figs - dietary Fluorescence Fostering Freezing Funding Future Gel Gene Expression Gene Proteins Gene Targeting Gene-Modified Generations Genes Genetic Genetic Recombination Genetically Modified Animals Genome Genomics Genotype Germ Cells Glycine Grant Health Hour Human Human Resources Individual Injection of therapeutic agent Inner Cell Mass Joints Knock-out Knockout Mice Lipids Lipoproteins Location Macrophage Colony-Stimulating Factor Maintenance Mediating Messenger RNA Metabolism Methods Microinjections Microsatellite Repeats Modeling Modification Molecular Biology Mothers Mouse Strains Mus Mutant Strains Mice Mutate Mutation Nature Neomycin Neomycin resistance gene Northern Blotting Nucleic Acids Occupations Operative Surgical Procedures Pattern Persons Phenols Plasmid Cloning Vector Plasmids Polymerase Chain Reaction Postdoctoral Fellow Precipitation Principal Investigator Procedures Process Production Proline Proteins Proto-Oncogenes Protocols documentation Publications Published Comment Reaction Replication Origin Reporter Reporter Genes Research Research Personnel Reverse Transcriptase Polymerase Chain Reaction Sampling Scanning Screening procedure Sepharose Services Simplexvirus Site Southern Blotting Specific qualifier value Speed Stem cells Stream Structural Genes Susceptibility Gene Systemic Lupus Erythematosus TK Gene Techniques Technology Temperature Terminator Codon Testing Tetanus Helper Peptide Tetracycline Resistance Thymidine Kinase Tissues Transfection Transgenes Transgenic Mice Transgenic Organisms Uterus VWF gene Vascular Endothelial Growth Factor Receptor-2 Western Blotting Wild Type Mouse Work Yang analog antibiotic G 418 base blastocyst cadherin 5 cell type congenic cost court data management density design design and construction egg embryonic stem cell experience fialuridine genome wide association study heme oxygenase-1 hindbrain homologous recombination interest macrophage male mouse model mutant novel null mutation programs promoter protein expression receptor research study response restriction enzyme stem success transmission process vector

项目摘要

Core C is a continuing core for Trangenic and Gene Targeting of Mice. In the next grant cycle Core C will assist Project 1 in constructing endothelial specific knockouts of SCAP and STATS on the LDL receptor null background.Core C will assist Projects 1 arid 2 in constructing HO-1 conditional and endothelial specific, knockout of HO-1 on the apoE null background. Core C will assist Project 3 in construction of a MGP-P64Q mutant mouse through gene targeting and will assist Project 3 in the generation of Abcc6-Wnt-promoterbeta- gal reporter double transgenic mice. Core C will provide consultation on breeding and colony management of transgenic mouse models to Project 4. Core C will assist Project 5 construction of, transgenci mice for testing atherosclerosis-related candidate genes. Core C will assist Project 6 with the construction of transgenic mice expressing dominant negatie NR4A receptor in macrophages. Core C will , also provide assistance to Core A in the characterization of primary cultues of mouse aortic endothelial cells by performing qPCR and Western blot analysis.These functions of Core C are essential to ensuring the efficient and cost-effective completion of the specific aims of all of the component Projects.

核心C是小鼠跨齿和基因靶向的持续核心。在下一个赠款周期中，核心C将协助项目1构建LDL受体NULL上的SCAP和统计数据的内皮特异性敲除背景Corcor C将协助项目1 ARID 2来构建HO-1条件和内皮特异性，在apoe null背景上敲除ho-1。 Core C将协助项目3构建MGP-P64Q 突变小鼠通过基因靶向，并将协助项目3的生成 GAL报告基因双转基因小鼠。 C核心将提供有关育种和殖民地的咨询对项目4的转基因鼠标模型的管理。CoreC将协助项目5的构建，用于测试与动脉粥样硬化相关的候选基因的基因小鼠。核心C将协助项目6 在巨噬细胞中表达主导性NR4A受体的转基因小鼠的结构。核心将还为核心A提供了辅助，以表征小鼠主动脉内皮细胞的原发性培养基通过执行QPCR和Western印迹分析。这些核心C的功能对于确保确保所有组件项目的具体目标有效且具有成本效益的完成。