An LTQ-FT Mass Spectrometer for Shotgun Proteomics

用于鸟枪蛋白质组学的 LTQ-FT 质谱仪

• 批准号: 6877316
• 负责人: Michael MacCoss
• 金额: $ 50万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877316
• 关键词: biomedical equipment purchase; biotechnology; mass spectrometry; proteomics

DESCRIPTION (provided by applicant): The enclosed proposal is requesting funds to expand the mass spectrometry and proteomics resources at the University of Washington through the purchase of a ThermoFinnigan LTQ-FT mass spectrometer. This instrument will be invaluable in the successful completion of our existing NIH funded projects involving the qualitative and quantitative analysis of proteins and protein modifications of complex mixtures. Furthermore, a number of our projects are extending quantitative proteomics technology to the measurement of changes in the rate of protein expression and degradation (protein dynamics). Integral to the timely completion of these NIH research projects is the availability of a mass spectrometer capable of i) sub 2 ppm mass accuracy to distinguish between similar mass peptides, ii) a dynamic range of >1000 in a single spectrum, iii) data-dependent acquisition of tandem mass spectra with the sensitivity and speed of a quadrupole ion trap, and iv) the ability to resolve and detect unusual isotope distributions of multiply charged peptides within a complex mixture. No such shared instrument is available to investigators at the University of Washington and several of our Users travel to outside laboratories to complete their NIH funded projects. Although we have managed to work around the lack of availability of a mass spectrometer capable of these measurements we cannot continue in this way if we hope to remain productive and competitive. We propose to address these limitations through the acquisition of a shared hybrid mass spectrometer which combines the robustness and speed of a linear quadrupole ion-trap with the resolution, mass accuracy, and dynamic range of a Fourier transform ion cyclotron mass spectrometer. The performance of the requested instrumentation will allow the faculty to address current technological limitations on a global level and will provide a resource that is not available within the university.

描述（由申请人提供）：所附提案请求资金通过购买 ThermoFinnigan LTQ-FT 质谱仪来扩大华盛顿大学的质谱和蛋白质组学资源。该仪器对于成功完成我们现有的 NIH 资助项目至关重要，这些项目涉及蛋白质的定性和定量分析以及复杂混合物的蛋白质修饰。此外，我们的许多项目正在将定量蛋白质组学技术扩展到测量蛋白质表达和降解速率的变化（蛋白质动力学）。及时完成这些 NIH 研究项目的一个重要因素是质谱仪的可用性，该质谱仪能够 i) 低于 2 ppm 的质量精度来区分相似质量的肽，ii) 单光谱中 >1000 的动态范围，iii) 数据-具有四极离子阱的灵敏度和速度的串联质谱的依赖采集，以及 iv) 解析和检测复杂混合物中多电荷肽的异常同位素分布的能力。华盛顿大学的研究人员没有这样的共享仪器，我们的一些用户前往外部实验室完成他们的 NIH 资助项目。尽管我们已经设法解决缺乏能够进行这些测量的质谱仪的问题，但如果我们希望保持生产力和竞争力，我们就不能继续这种方式。我们建议通过购买共享混合质谱仪来解决这些限制，该质谱仪将线性四极杆离子阱的鲁棒性和速度与傅里叶变换离子回旋质谱仪的分辨率、质量精度和动态范围结合起来。所需仪器的性能将使教师能够解决全球范围内当前的技术限制，并将提供大学内无法获得的资源。