Genes underlying reproductive behavior and physiology

生殖行为和生理学的基因

• 批准号: 7144516
• 负责人: MICHELLE N ARBEITMAN
• 金额: $ 28.24万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7144516
• 关键词: Drosophilidae; RNA interference; arthropod genetics; behavioral /social science research tag; behavioral genetics; functional /structural genomics; gender difference; gene expression; genetic regulation; morphology; neurogenetics; neurophysiology; neuropsychology; protein structure function; sex behavior; sex linked trait; transcription factor

DESCRIPTION (provided by applicant): A fundamental problem in neurobiology is understanding how the potential for complex behaviors are established. Drosophila melanogaster is an excellent model system to explore this question, given that a robust behavior, mating behavior, is genetically programmed. Drosophila male courtship consists of a series of steps directed towards a female. Females that have not been mated are receptive to male courtship behaviors, whereas those that have been recently mated will reject these advances. There is molecular-genetic evidence demonstrating that the sex hierarchy plays a central role in establishing courtship and mating behaviors in both males and females, through the action of transcription factors encoded by doublesex (dsx) and fruitless (fru). Both dsx and fru produce sex-specific isoforms. Molecular-genetic analyses have demonstrated that both dsx and fru play fundamental roles in the establishment of sex-specific cell fates important for reproductive behaviors. Despite the importance of these transcription factors, practically nothing is known about their targets. We will use genomic approaches to identify genes regulated by these transcription factors. We will study the functions of these target genes in the nervous system to gain insight into the genetic specification of behavior. This will provide a foundation for understanding the specification of complex behaviors in other organisms, including humans, for which we have very limited understanding.

描述（由申请人提供）：神经生物学的一个基本问题是理解复杂行为的潜力是如何建立的。果蝇是探索这个问题的优秀模型系统，因为强大的行为（交配行为）是通过基因编程的。雄性果蝇的求爱包括一系列针对雌性的步骤。尚未交配的雌性会接受雄性的求爱行为，而最近交配过的雌性会拒绝这些行为。分子遗传学证据表明，通过双性（dsx）和无果（fru）编码的转录因子的作用，性别等级在雄性和雌性的求偶和交配行为的建立中发挥着核心作用。 dsx 和 fru 均产生性别特异性亚型。分子遗传学分析表明，dsx 和 fru 在建立对生殖行为重要的性别特异性细胞命运方面发挥着重要作用。尽管这些转录因子很重要，但实际上对其靶标一无所知。我们将使用基因组方法来识别受这些转录因子调控的基因。我们将研究这些目标基因在神经系统中的功能，以深入了解行为的遗传规范。这将为理解包括人类在内的其他生物体的复杂行为规范提供基础，而我们对此的了解非常有限。