Genes underlying reproductive behavior and physiology

生殖行为和生理学的基因

• 批准号: 7888951
• 负责人: MICHELLE N ARBEITMAN
• 金额: $ 32.51万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-03 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7888951
• 关键词: Address; Adult; Antibodies; Behavior; Behavioral; Biological Models; Cells; Classification; Complex; Courtship; Development; Drosophila genus; Drosophila melanogaster; Female; Foundations; Gene Targeting; Genes; Genetic; Genomics; Goals; Human; In Situ; Molecular; Molecular Genetics; Morphology; Nervous system structure; Neuraxis; Neurobiology; Neurons; Organism; Partner in relationship; Pathway interactions; Pattern; Physiological; Physiology; Play; Positioning Attribute; Production; Protein Isoforms; RNA; RNA Interference; RNA Splicing; Reproductive Behavior; Role; Series; Specific qualifier value; Staging; Testing; Tissues; Transgenic Organisms; base; behavior change; genetic analysis; insight; mRNA Precursor; male; programs; sex; sex determination; transcription factor

A fundamental problem in neurobiology is understanding how the potential for complex behaviors are established. Drosophila melanogaster is an excellent model system to explore this question, given that a robust behavior, mating behavior, is genetically programmed. Drosophila male courtship consists of a series of steps directed towards a female. Females that have not been mated are receptive to male courtship behaviors, whereas those that have been recently mated will reject these advances. There is molecular- genetic evidence demonstrating that the sex hierarchy plays a central role in establishing courtship and mating behaviors in both males and females, through the action of transcription factors encoded by doublesex (dsx) and fruitless (fru). Both dsx and fru produce sex-specific isoforms. Molecular-genetic analyses have demonstrated that both dsx and fru play fundamental roles in the establishment of sex- specific cell fates important for reproductive behaviors. Despite the importance of these transcription factors practically nothing is known about their targets. We will use genomic approaches to identify genes regulated by these transcription factors. We will study the functions of these target genes in the nervous system to gain insight into the genetic specification of behavior. This will provide a foundation for understanding the specification of complex behaviors in other organisms, including humans, for which we have very limited understanding.

神经生物学的一个基本问题是理解复杂行为的潜力如何 已确立的。果蝇是探索这个问题的一个很好的模型系统，因为 鲁棒的行为，即交配行为，是由基因编程的。果蝇雄性求爱由一系列 指向女性的步骤。尚未交配的雌性会接受雄性求爱 行为，而那些最近交配过的人会拒绝这些进步。有分子- 遗传证据表明，性别等级在求爱和求爱的建立中起着核心作用 通过转录因子的作用，雄性和雌性的交配行为 双性（dsx）和无果（fru）。 dsx 和 fru 均产生性别特异性亚型。分子遗传学 分析表明，dsx 和 fru 在建立性关系中发挥着重要作用。 对生殖行为很重要的特定细胞命运。尽管这些转录因子很重要 几乎对他们的目标一无所知。我们将使用基因组方法来识别受调控的基因 通过这些转录因子。我们将研究这些靶基因在神经系统中的功能 深入了解行为的遗传规范。这将为理解 其他生物体（包括人类）复杂行为的规范，对此我们的了解非常有限 理解。