Multi-Parameter Flow Cytometer with High-Speed Sorting

具有高速分选功能的多参数流式细胞仪

• 批准号: 6729502
• 负责人: Alice L. Givan
• 金额: $ 39.74万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6729502
• 关键词: biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant application is for funding for the purchase of a Becton Dickinson FACSAria high-speed sorting flow cytometer. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a shared resource of the Norris Cotton Cancer Center at Dartmouth Medical School. The new flow cytometer will provide users with the up-to-date sorting and analysis capabilities that they require. The requirement for a new flow cytometer is based on several needs that are not met by the cytometers currently available at Dartmouth. The primary need is for the ability to define cells by more than four fluorescence parameters. The FACSAria will have three lasers and will permit users to excite a range of fluorochromes across the spectrum and to analyze and sort cells on any (or all) of thirteen fluorescence parameters. In addition, the FACSAria will allow users to save time and cell sample by the sorting of four populations of cells at one time; will allow cross-beam compensation to facilitate the use of overlapping fluorochromes; will facilitate the definition of cells with non-rectangular sort gates; and will provide for more efficient sorting at high cell flow rates. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and imaging supervisor. Running costs are met by a subsidy from the NCI core grant to the Norris Cotton Cancer Center, by hourly charges to users, and by institutional support from Dartmouth Medical School. Additional institutional support will be provided by Dartmouth Medical School toward the purchase cost of the FACSAria. At the present time, there are 47 NIH grant holders dependent upon the flow cytometers in the Englert Core Laboratory; they hold, as PIs, 88 NIH grants. A "major user group" has been identified. These nine major users project 78% of the total use of the FACSAria cytometer. They hold, as PIs, NIH grants.

描述（由申请人提供）：此共享仪器拨款申请旨在资助购买 Becton Dickinson FACSAria 高速分选流式细胞仪。该仪器将集成到恩格勒特细胞分析实验室，该实验室是达特茅斯医学院诺里斯科顿癌症中心的共享资源。新型流式细胞仪将为用户提供他们所需的最新分类和分析功能。对新型流式细胞仪的要求是基于达特茅斯目前可用的细胞仪无法满足的多种需求。主要需求是能够通过四个以上荧光参数来定义细胞。 FACSAria 将配备三个激光器，允许用户在整个光谱范围内激发一系列荧光染料，并根据 13 个荧光参数中的任何一个（或全部）对细胞进行分析和分类。此外，FACSAria 将允许用户通过一次分选四个细胞群来节省时间和细胞样本；将允许交叉光束补偿，以方便重叠荧光染料的使用；将有助于定义具有非矩形排序门的单元格；并将在高细胞流速下提供更有效的分选。恩格勒特细胞分析实验室在一名主任、一名联合主任和一个顾问委员会的控制下运作，并得到流量和成像主管的技术支持。运行成本由 NCI 向 Norris Cotton 癌症中心提供的核心拨款、向用户按小时收费以及达特茅斯医学院的机构支持来支付。达特茅斯医学院将提供额外的机构支持，用于支付 FACSAria 的购买费用。目前，有 47 名 NIH 资助者依赖 Englert 核心实验室的流式细胞仪；作为 PI，他们拥有 88 项 NIH 拨款。已经确定了“主要用户组”。这九个主要用户占 FACSAria 细胞仪总使用量的 78%。作为 PI，他们持有 NIH 拨款。