Laser Scanning Cytometer for Purdue University

普渡大学激光扫描细胞仪

• 批准号: 6876911
• 负责人: JOSEPH PAUL ROBINSON
• 金额: $ 34.2万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876911
• 关键词: biomedical equipment purchase; biomedical facility; biomedical resource; flow cytometry; health science research support; molecular /cellular imaging

DESCRIPTION (provided by applicant): Flow cytometry capabilities have been maturing at Purdue University over the past 15 years. Since 1987, we have upgraded our flow cytometers and imaging instruments including support from SIG (1993, 2000, 2001) which allowed us to maintain high quality services and instruments to the faculty of the university. Since 1988, the Purdue University Cytometry Laboratories (PUCL) has maintained continuous service to the faculty. Over 366 faculty have used the facility in the past 15 years. Over 300 graduate students have been trained at advanced levels. The PUCL facility however, is not just about providing instrument support to researchers. Once the instruments are in place, we provide excellent technical support and quality advice. We maintain 4 full time technicians and a software expert whose entire role is to provide quality support to investigators. Instrumentation that comes into this facility is guaranteed to be not only available to faculty, but is provided a la carte with a commitment to quality service. We successfully introduced flow cytometry to many faculty who were not aware of its capability and supported those who have driven our program by participating in our SIG grants. We now find ourselves challenged by the significant development of cytomics - functional analysis with a higher need for parameterized morphology and sub-cellular localization and gene expression on cells in cell cultures and in tissue while maintaining spatial correlations. The Compucyte iCyte Imaging Cytometer is a commercially available cytometer that offers this capability for which this application requests funding. Although driven by 4 primary and 6 secondary users, our track record predicts that it will be used by many more faculty as our experience in its operation and management matures. Placement of a shared resource into a facility that has a strong track record of sharing and support is a cost effective way of supporting NIH funded researchers at this institution.

描述（由申请人提供）：普渡大学的流式细胞术能力在过去 15 年中不断成熟。自 1987 年以来，我们升级了流式细胞仪和成像仪器，包括 SIG 的支持（1993 年、2000 年、2001 年），这使我们能够为大学教师提供高质量的服务和仪器。自 1988 年以来，普渡大学细胞计数实验室 (PUCL) 一直为教师提供持续的服务。在过去 15 年里，超过 366 名教职员工使用过该设施。超过300名研究生接受了高级培训。然而，PUCL 设施不仅仅是为研究人员提供仪器支持。一旦仪器到位，我们将提供出色的技术支持和质量建议。我们拥有 4 名全职技术人员和一名软件专家，其全部职责是为调查人员提供质量支持。进入该设施的仪器不仅保证可供教职员工使用，而且是按菜单提供的，并承诺提供优质服务。我们成功地将流式细胞术介绍给许多不了解流式细胞术功能的教师，并通过参与我们的 SIG 资助来支持那些推动我们项目的人。我们现在发现自己面临着细胞组学显着发展的挑战，即功能分析对细胞培养物和组织中细胞的参数化形态、亚细胞定位和基因表达的更高需求，同时保持空间相关性。 Compucyte iCyte 成像细胞仪是一款市售细胞仪，它提供了本申请要求资助的此功能。尽管由 4 个主要用户和 6 个次要用户推动，但我们的跟踪记录预测，随着我们运营和管理经验的成熟，它将被更多的教师使用。将共享资源放入具有良好共享和支持记录的设施中是支持该机构 NIH 资助的研究人员的一种经济高效的方式。