Purchase of Leica TCS SP2 Confocal Microscope System

购买Leica TCS SP2共焦显微镜系统

• 批准号: 6439974
• 负责人: Alice L. Givan
• 金额: $ 36.8万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439974
• 关键词: biomedical equipment purchase; cell biology; confocal scanning microscopy

This Shared Instrumentation Grant application is for the purchase of a Leica TCS SP2 laser scanning confocal microscope system. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a Shared Core Resource of the Norris Cotton Cancer Center at Dartmouth Medical School. The Englert Laboratory was establish3d in 1981 for flow cytometry and has been providing Dartmouth with imaging as well as flow cytometric instrumentation since 1993. The new confocal instrument will replace an outdated confocal system, giving users access to the stable, flexible, and up-to-date imaging capabilities that they require. The requirement for a new confocal system is based on several urgent needs. The existing confocal system does not have the laser stability or excitation and emission flexibility required for multicolor quantitative work by a varied and large group of users. In addition, there is an expressed need for live cell studies that cannot be met by the existing upright microscope stand lacking temperature control. Finally, a new system will have the ability to acquire images with 12 bit intensity resolution, will provide regions of interest for FRAP and inverse FRAP analysis, and will provide 4 PMTs for four color fluorescence acquisition-all of which are lacking on our current system and are required now by many users. A Leica TCS SP2 system will satisfy current users of the Core Facility and will provide flexibility for adaptation to the needs of users in the future. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and an imaging supervisor. It is supported financially from the NCI core grant to the Norris Cotton Cancer Center, by hourly charges to users, and by institutional support from Dartmouth Medical School. At the present time, there are 33 NIH grant holders dependent upon the confocal system in the Englert Core Laboratory; they hold, as PIs, 50 NIH grants, with $9,918,884 in annual direct costs. A "major user group" has been identified. These eleven major users project 84% of the total use of the confocal system. They hold, as PIs, 20 NIH grants with $5,161, 571 in annual direct costs.

该共享仪器补助金申请用于购买 Leica TCS SP2 激光扫描共焦显微镜系统。该仪器将集成到恩格勒特细胞分析实验室，该实验室是达特茅斯医学院诺里斯·科顿癌症中心的共享核心资源。 Englert 实验室成立于 1981 年，专门从事流式细胞术，自 1993 年以来一直为达特茅斯提供成像和流式细胞术仪器。新的共焦仪器将取代过时的共焦系统，使用户能够获得稳定、灵活和最新的技术-他们需要的最新成像能力。对新共焦系统的需求是基于几个紧迫的需求。现有的共焦系统不具备由不同的大量用户进行多色定量工作所需的激光稳定性或激发和发射灵活性。此外，对活细胞研究的明确需求是缺乏温度控制的现有直立显微镜支架无法满足的。最后，新系统将能够采集 12 位强度分辨率的图像，为 FRAP 和反 FRAP 分析提供感兴趣区域，并为四色荧光采集提供 4 个 PMT——所有这些都是我们当前系统所缺乏的现在很多用户都需要。 Leica TCS SP2 系统将满足核心设施的当前用户，并将提供灵活性以适应未来用户的需求。恩格勒特细胞分析实验室在一名主任、一名联合主任和一个顾问委员会的控制下运作，并得到流程和成像主管的技术支持。它的财政支持来自 NCI 向 Norris Cotton 癌症中心提供的核心拨款、按小时向用户收费以及达特茅斯医学院的机构支持。目前，有 33 名 NIH 资助者依赖 Englert 核心实验室的共聚焦系统；作为 PI，他们拥有 50 项 NIH 拨款，每年直接费用为 9,918,884 美元。已经确定了“主要用户组”。这 11 个主要用户占共焦系统总使用量的 84%。作为 PI，他们拥有 20 项 NIH 拨款，其中 5,161 美元，每年直接费用为 571 美元。