FLOW CYTOMETRY AND CELL IMAGING SHARED RESOURCE

流式细胞术和细胞成像共享资源

基本信息

批准号：
7944637
负责人：
Alice L. Givan
金额：
$ 12.12万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-21 至 2013-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944637
关键词：
3-Dimensional Argon Biological Assay Calcium Calibration Cancer Center Cancer Center Support Grant Cell Cycle Cell physiology Cells Collaborations Color Comb animal structure Communication Computer software Computers Consult Data Data Analyses Development Documentation Elements Equipment Flow Cytometry Fluorescence Foundations Funding Future Goals Gossypium Grant Hour Image Image Analysis Immunophenotyping Individual Kinetics Laboratories Lasers Leukocytes Measures Methods Microscope Mission Monitor National Center for Research Resources Pattern Preparation Quality Control Research Research Personnel Resource Sharing Resources Running Scanning Schedule Scientist Services Side Signal Transduction Sorting - Cell Movement Speed Staging Students Surveys System Techniques Technology Temperature Time Tissues Training Work assay development cellular imaging detector digital imaging fluorescence microscope instrument instrumentation interest medical schools programs reconstruction ward

项目摘要

A Flow Cytometry Laboratory was established as a Shared Resource for Morris Cotton Cancer Center in 1981 with a grant from the Fannie E. Rippel Foundation for the purchase of an Ortho System 50 Cytofluorograf. That instrument was replaced in March 1991 by two Becton Dickinson flow cytometers purchased with a grant from the Shared Instrumentation Grant Program (SIG) of the National Center for Research Resources (NIH). Between 1993 and 1995, imaging capability was added to the Flow Cytometry Resource with the acquisition of a confocal imaging system with funding from the SIG program. This equipment established the Flow Cytometry and Cell Imaging Shared Resource to provide both flow and image analysis for NCCC. Since the last application for Core grant renewal, the Shared Resource has received funding from the NIH SIG program, for the purchase of a BD FACSAria sorting flow cytometer and a BD FACSCanto benchtop flow cytometer, and from Dartmouth Medical School for the purchase of a Zeiss stereo fluorescence microscope system. In addition, we have been able to acquire five Macintosh computers and have placed them in a new room to provide users with comfortable access to software for data analysis. At the present time, the Flow Cytometry and Cell Imaging Shared Resource contains four benchtop flow cytometers (two FACScans, a FACSCalibur, and a FACSCanto), two sorting flow cytometers (a FACStar Plus and a FACSAria), a Zeiss 510 Meta laser scanning confocal system, a Compucyte laser scanning cytometer, a Zeiss stereo fluorescence microscope, and a Zeiss fluorescence inverted microscope. In addition, there are six Macintosh workstations and one PC workstation for analysis of data from the flow and imaging instruments. The Flow Cytometry and Cell Imaging Shared Resource is located in a suite of seven rooms in the Borwell Research Building adjacent to the Rubin Building. It is directed by Dr. Alice L. Givan, with Dr. Paul M. Guyre as co-director. Mr. Gary Ward and Mr. Kenneth Orndorff are supervisors of the flow and imaging instruments, respectively. The Shared Resource is being used by 79 Pis, with approximately 300 users from those laboratories. It is funded by a combination of subsidy from the CCSG and chargebacks to individual users.

流式细胞术实验室作为莫里斯科顿癌症中心的共享资源在 1981 年，Fannie E. Rippel 基金会拨款购买 Ortho System 50 细胞荧光照片。该仪器于 1991 年 3 月被两台 Becton Dickinson 流式细胞仪取代由国家中心的共享仪器资助计划 (SIG) 资助购买研究资源（NIH）。 1993 年至 1995 年间，流式细胞术增加了成像功能在 SIG 计划的资助下购买共焦成像系统的资源。这设备建立了流式细胞术和细胞成像共享资源，以提供流式细胞仪和细胞成像技术 NCCC 的图像分析。自上次核心拨款更新申请以来，共享资源已获得 NIH SIG 计划的资助，用于购买 BD FACSAria 分选流式细胞仪 BD FACSCanto 台式流式细胞仪，并从达特茅斯医学院购买 Zeiss 立体荧光显微镜系统。此外，我们还购买了五台 Macintosh 电脑并将它们放置在一个新房间中，以便用户可以轻松地访问数据分析软件。目前，流式细胞术和细胞成像共享资源包含四个台式流细胞计数仪（两台 FACScan、一台 FACSCalibur 和一台 FACSCanto）、两台分选流式细胞仪（一台 FACStar Plus 和 FACSAria）、Zeiss 510 Meta 激光扫描共焦系统、Compucyte 激光扫描细胞仪、蔡司体视荧光显微镜、蔡司荧光倒置显微镜。在此外，还有六台Macintosh工作站和一台PC工作站，用于分析来自流程和数据的数据。成像仪器。流式细胞术和细胞成像共享资源位于一套由七个位于鲁宾大楼附近的博韦尔研究大楼的房间。它由 Alice L. Givan 博士执导， Paul M. Guyre 博士担任联合主任。 Gary Ward先生和Kenneth Orndorff先生担任流程主管和成像仪器，分别。共享资源正在被 79 个 Pi 使用，其中大约有来自这些实验室的 300 位用户。它的资金来自 CCSG 的补贴和退款给个人用户。