Laser Scanning Confocal Microscope

激光扫描共焦显微镜

• 批准号: 6877450
• 负责人: RALPH A. NIXON
• 金额: $ 43.69万
• 依托单位: NATHAN S. KLINE INSTITUTE FOR PSYCH RES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877450
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; confocal scanning microscopy; molecular /cellular imaging

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Zeiss LSM510 laser confocal microscope. There is a strong justification for a new confocal laser microscope to replace our existing largely outdated model that currently serves all of the NIH-funded researchers at our Institute. The new microscope will extend the capabilities of our imaging facility, while providing a much-needed and valuable research tool for the seven applicant investigators and their co-investigators, which form the major user group. Instrumentation comparable to that requested is not available near NKI. The analysis and interpretation of structural and cell biological data by the best technological means available, including laser confocal microscopy, is of critical importance to the continuation and enhancement of ongoing research programs, and to the evolution of these programs in new directions. Acquisition of the new instrument will enable scientists throughout our institute to explore a diversity of topics organized around the theme of basic and translational neuroscience of neurodegenerative and neuropsychiatric diseases. All of the NIH-funded programs referenced in the application have components that require and would be strengthened by confocal laser microscopy. These are summarized as follows: 1) AD-related proteins following therapeutic intervention in animal models; 2) Expression Profiling in Neurodegenerative Disorders; 3) Cystatin C in cerebral hemorrhage and in Alzheimer's disease (AD); 4) The endocytic pathway in AD and Down's Syndrome; 5) The endosomal-autophagic-lysosomal system in AD Pathogenesis; 6) The role of disrupted cholesterol metabolism in AD pathogenesis; and 7) Signal Transduction in Neuropsychiatric Disease. An enhancement of the Institute's imaging capabilities will allow all applicant investigators to pursue funded research programs with greater efficiency and depth, to expand existing programs in new directions and to develop new programs.

描述（由申请人提供）： 申请资金购买蔡司 LSM510 激光共焦显微镜。有充分理由使用新型共焦激光显微镜来取代我们现有的基本上过时的模型，该模型目前为我们研究所的所有 NIH 资助的研究人员提供服务。新的显微镜将扩展我们成像设施的功能，同时为构成主要用户群体的七名申请研究人员及其共同研究人员提供急需且有价值的研究工具。 NKI 附近没有与要求相当的仪器。通过现有的最佳技术手段（包括激光共聚焦显微镜）对结构和细胞生物学数据进行分析和解释对于持续和加强正在进行的研究项目以及这些项目向新方向的发展至关重要。购买新仪器将使我们研究所的科学家能够探索围绕神经退行性和神经精神疾病的基础和转化神经科学主题组织的各种主题。申请中引用的所有 NIH 资助的项目都需要共焦激光显微镜，并且可以通过共焦激光显微镜得到加强。这些总结如下：1）动物模型中治疗干预后的AD相关蛋白； 2) 神经退行性疾病的表达谱； 3）胱抑素C在脑出血和阿尔茨海默病（AD）中的作用； 4）AD和唐氏综合症的内吞途径； 5) AD发病机制中的内体-自噬-溶酶体系统； 6) 胆固醇代谢紊乱在 AD 发病机制中的作用； 7) 神经精神疾病中的信号转导。 研究所成像能力的增强将使所有申请研究人员能够以更高的效率和深度开展受资助的研究项目，将现有项目扩展到新的方向并开发新项目。