BD LSR II MULTIPARAMETER FLOW CYTOMETER

BD LSR II 多参数流式细胞仪

• 批准号: 6876431
• 负责人: NITIN J KARANDIKAR
• 金额: $ 32.84万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876431
• 关键词: biomedical equipment purchase; flow cytometry; immune system; immunology; immunopathology

DESCRIPTION (provided by applicant): The objectives of this proposal are to purchase a state-of-the-art multichromatic flow cytometer in order to support, enhance and fulfill the specialized technologic needs of a group of multiple NIH-funded researchers at UT Southwestern. The major user group conducts research on varied areas of immunology and requires the proposed instrument to be able to gain and enhance scientific insight into the questions being addressed in ongoing projects. As our understanding of the immunologic basis of various disease situations grows, it is becoming increasingly clear that populations of immune cells are not homogeneous and need to be studied as distinct subpopulations in order to understand their precise role or to modulate their function in a therapeutic setting. Several functional assays can now be performed by flow cytometric technology with a distinct advantage over traditional methodologies. These applications are changing our understanding of the interplay between various cell types of the immune system. In addition, powerful utilization and analysis of multiparametric data can be attained through bioinformatics. The addition of the 4- laser, 10-color flow cytometer will provide cutting-edge instrumentation that will dramatically enhance research across the user group. We present here the justification and rationale for our proposal, cite various specific examples of how the instrument would provide greater insight into ongoing NIH-funded research and outline the strong institutional commitment that will enable the smooth implementation of the NCRR SIG program.

描述（由申请人提供）：本提案的目标是购买一台最先进的多色流式细胞仪，以支持、增强和满足 UT 西南大学由多个 NIH 资助的研究人员组成的小组的专业技术需求。主要用户群体对免疫学的各个领域进行研究，并要求拟议的仪器能够获得并增强对正在进行的项目中正在解决的问题的科学见解。随着我们对各种疾病情况的免疫学基础的了解不断加深，越来越清楚的是，免疫细胞群不是同质的，需要作为不同的亚群进行研究，以便了解它们的精确作用或在治疗环境中调节它们的功能。现在可以通过流式细胞术技术进行多种功能测定，与传统方法相比具有明显的优势。这些应用正在改变我们对免疫系统各种细胞类型之间相互作用的理解。此外，通过生物信息学可以实现多参数数据的强大利用和分析。 4 激光、10 色流式细胞仪的添加将提供尖端仪器，从而极大地增强整个用户组的研究。我们在此介绍我们提案的理由和理由，引用各种具体例子来说明该工具如何为正在进行的 NIH 资助的研究提供更深入的了解，并概述强有力的机构承诺，以确保 NCRR SIG 计划的顺利实施。