BD FACSAria Flow Cytometer Cell Sorter

BD FACSAria 流式细胞仪细胞分选仪

基本信息

批准号：
6877610
负责人：
CHOU-ZEN GIAM
金额：
$ 37.76万
依托单位：
HENRY M. JACKSON FDN FOR THE ADV MIL/MED
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-02-15 至 2006-02-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877610
关键词：
biomedical equipment purchase cell biology cytology flow cytometry

项目摘要

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant proposal is for the purchase of the BD FACSAria Flow Cytometer Cell Sorter. This is a state-of-the-art fully digital, three laser (488nm, 633nm and 407nm) Flow Cytometer Cell Sorter. It is capable of sorting two to four populations at an acquisition rate of up to 70,000 cells per second. This request includes several options. The water recirculator will provide optimal temperature control for the sample and sorted fractions. The Automated Cell Deposition Unit (ACDU) option is an integrated cell deposition system for multi-well plate and slide sorting. The aerosol management system option will enable sorting of biologically hazardous samples. The HP XW4000 Workstation provides a powerful data management system. The new BD FACSAria cell sorter will be housed at USUHS in the Biomedical Instrumentation Center (BIC), Flow Cytometry Facility, Room G235, and will be operated and maintained by the Flow Cytometry Facility Director, Karen M. Wolcott. The Principal Investigator, William C. Gause, will provide administrative/scientific oversight in consultation with an internal advisory committee. The BD FACSAria is requested as a replacement for the current flow cytometer cell sorter, a 13-year old Beckman Coulter EPICS ELITE ESP. Many requests for sorting have been declined due to the inability of this antiquated instrument to support the needs of many research protocols, especially those of the NIH-supported investigators identified in this grant. First priority will be given to the NIH-supported scientists who are participating in this grant proposal. They include: 1.) Brumeanu, Teodor D. - Prevention of Type 1 Diabetes by soluble MHC II- peptides; 2.) Cutler, Mary Lou - Role of Ras Effectors in Mammary Differentiation; 3.) Darling, Thomas N. - Tumorigenesis in Tuberous Sclerosis; 4.) Gause, William C. - Regulation of Th2 cell commitment, differentiation, and cell cycle progression in vivo; 5.) Giam, Chou-Zen - Molecular Biology and Pathogenesis of HTLV-1; 6.) Pollard, Harvey B. - Regulation of Inflammatory Pathways in Cystic Fibrosis; 7.) Provencio, Ignacio Isolation and Characterization of Melanopsin-Containing Retinal Ganglion Cells; 8.) Schaefer, Brian C. - Antigen receptor regulation of NF-KB signaling in lymphocyte activation and tumorigenesis; 9.) Snapper, Clifford M. - Dendritic and T cells in antibacterial Ig responses; 10.) Tsokos, George C. - Regulation of lymphocyte function during Systemic Lupus Erythematosus (SLE); and 11.) Via, Charles S. - Immunopathogenesis of Lupus. It is anticipated that this group of investigators will require 95% of the total usage of the instrument. The remaining 5% will be allocated to USUHS, NIH-supported research scientists or other individuals in the USUHS community on a first come, first served basis. Sorting time may be made available to investigators outside of the USUHS community from time to time when the instrument would otherwise not be used.

描述（由申请人提供）：此共享仪器拨款提案用于购买 BD FACSAria 流式细胞仪细胞分选仪。这是一款最先进的全数字三激光（488nm、633nm 和 407nm）流式细胞仪细胞分选仪。它能够以高达每秒 70,000 个细胞的采集速率对 2 到 4 个细胞群进行分选。该请求包括多个选项。水再循环器将为样品和分选的级分提供最佳的温度控制。自动细胞沉积装置 (ACDU) 选项是用于多孔板和载玻片分选的集成细胞沉积系统。气溶胶管理系统选项将能够对生物危险样品进行分类。 HP XW4000 工作站提供强大的数据管理系统。新的 BD FACSAria 细胞分选机将安装在 USUHS 生物医学仪器中心 (BIC) 流式细胞术设施 G235 室，并由流式细胞术设施主任 Karen M. Wolcott 操作和维护。首席研究员 William C. Gause 将与内部咨询委员会协商提供行政/科学监督。 BD FACSAria 被要求替代当前的流式细胞仪细胞分选仪（已有 13 年历史的 Beckman Coulter EPICS ELITE ESP）。由于这种过时的仪器无法支持许多研究方案的需求，尤其是本次拨款中确定的 NIH 支持的研究人员的需求，许多排序请求已被拒绝。首先将优先考虑参与本资助提案的 NIH 支持的科学家。它们包括： 1.) Brumeanu, Teodor D. - 通过可溶性 MHC II 肽预防 1 型糖尿病； 2.) Cutler, Mary Lou - Ras 效应器在乳腺分化中的作用； 3.) Darling, Thomas N. - 结节性硬化症的肿瘤发生； 4.) Gause, William C. - 体内 Th2 细胞定型、分化和细胞周期进展的调节； 5.) Giam, Chou-Zen - HTLV-1 的分子生物学和发病机制； 6.) Pollard, Harvey B. - 囊性纤维化中炎症途径的调节； 7.) Provencio、Ignacio 含有黑视蛋白的视网膜神经节细胞的分离和表征； 8.) Schaefer, Brian C. - 淋巴细胞激活和肿瘤发生中 NF-KB 信号传导的抗原受体调节； 9.) Snapper, Clifford M. - 抗菌 Ig 反应中的树突状细胞和 T 细胞； 10.) Tsokos, George C. - 系统性红斑狼疮 (SLE) 期间淋巴细胞功能的调节； 11.) Via, Charles S. - 狼疮的免疫发病机制。预计这组研究人员将需要仪器总使用量的 95%。剩余的 5% 将按照先到先得的原则分配给 USUHS、NIH 支持的研究科学家或 USUHS 社区中的其他个人。当仪器无法使用时，USUHS 社区之外的调查人员可能会不时获得分选时间。