FLOW CYTOMETRY FACILITY HIGH SPEED SORTER

流式细胞术设备高速分选机

• 批准号: 6877619
• 负责人: BICE PERUSSIA
• 金额: $ 25.67万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877619
• 关键词: biomedical equipment purchase; cell biology; cytology; flow cytometry

DESCRIPTION (provided by applicant): This proposal is to acquire a state-of-the-art flow cytometer with sorting capabilities in order to upgrade the multi-user Flow Cytometry Facility of the Kimmel Cancer Center, Thomas Jefferson University, and thus optimally support the research activities of the NIH-funded members of the Cancer Center. We request to purchase a Cytomation MoFlo(TM) HTS Ultra High-Speed Sort Flow Cytometer with capability of high speed 4-way sorting, of routine simultaneous analysis of greater than 4 fluorochromes, and with the added capability of single cell deposition. Currently, the KCC Flow Cytometry Facility is the sole facility of this type on the TJU campus. It is equipped with a 12-year old Coulter EPICS Elite ESP, which is also a sorting instrument, and two analyzers: a 4-year old Coulter EPICS XL-MCL, and a 15- year old Becton-Dickinson FACSCalibur Benchtop Analyzer. A newly acquired BD Aria Sorter is physically present (although yet to be installed in a newly to-be-developed facility) in the Neurology Department at TJU. Whenever in operation, however, this piece of equipment will be dedicated to the specific needs of Neurological Sciences research, with the KCC investigator having limited access. The increased number of KCC investigators requiring high-speed cell sorting in their NIH-sponsored projects serves as the main justification for our request for a Cytomation MoFlo in the existing and operating KCC facility. Our application is focused on the need of this core facility to upgrade its services as a result of: 1) our NIH-investigators' increasing requests for desirable cell yields of sorted samples in reasonable time periods, which are currently unachievable with the present equipment; 2) an increasing demand for rare-event sorting within large cell populations, and for simultaneous analysis of greater than 4 fluorochrome-detectable parameters; and 3) the lack of sufficient and reliable access to near-by high speed sorting services. The availability of the Cytomation MoFlo instrument requested would allow outstanding basic and clinical research to be continued, and new projects to be initiated, at the KCC. It also would increase the efficiency of the existing Facility, allowing reliable use of the instruments by more investigators, and by other investigators (not in need of the machine requested here) to perform the more routine analysis and sorting in a timely manner on the old equipment, without the lag existent at present.

描述（由申请人提供）：本提案旨在购买一台具有分选功能的最先进的流式细胞仪，以升级托马斯杰斐逊大学金梅尔癌症中心的多用户流式细胞仪设施，从而最佳地支持NIH 资助的癌症中心成员的研究活动。我们要求购买一台 Cytomation MoFlo(TM) HTS 超高速分选流式细胞仪，具有高速 4 路分选功能，能够对 4 种以上荧光染料进行常规同步分析，并具有单细胞沉积的附加功能。目前，KCC 流式细胞术设施是 TJU 校园内唯一的此类设施。它配备了一台 12 年机龄的 Coulter EPICS Elite ESP（也是一种分选仪）和两台分析仪：一台机龄 4 年的 Coulter EPICS XL-MCL 和一台机龄 15 年的 Becton-Dickinson FACSCalibur 台式分析仪。 TJU 神经内科实际配备了一台新购买的 BD Aria Sorter（尽管尚未安装在新开发的设施中）。然而，无论何时运行，该设备都将专门用于满足神经科学研究的特定需求，而 KCC 调查员的访问权限有限。在 NIH 资助的项目中需要高速细胞分选的 KCC 研究人员数量不断增加，这是我们要求在现有和运行的 KCC 设施中使用 Cytomation MoFlo 的主要理由。我们的申请重点是该核心设施需要升级其服务，因为：1）我们的 NIH 研究人员对在合理时间段内分选样品的理想细胞产量的要求不断增加，而目前的设备无法实现这一目标； 2) 对大细胞群中罕见事件分选以及同时分析超过 4 个荧光染料可检测参数的需求不断增长； 3) 缺乏足够且可靠的附近高速分拣服务。所需的 Cytomation MoFlo 仪器的可用性将使 KCC 能够继续进行出色的基础和临床研究，并启动新项目。它还将提高现有设施的效率，允许更多调查员和其他调查员（不需要此处请求的机器）可靠地使用仪器，以便对旧设备及时进行更常规的分析和分类。设备，不存在目前存在的滞后。