High content cell-based assay system

高内涵细胞检测系统

• 批准号: 6731624
• 负责人: RAYMOND J DINGLEDINE
• 金额: $ 42.87万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731624
• 关键词: bioassay; bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; clinical biomedical equipment; cytology; high throughput technology

DESCRIPTION (provided by applicant): This proposal to the National Center for Research Resources seeks funds to establish a state-of-the-art High Content Cell-based Screening System that would serve researchers in biomedical departments at the Emory University School of Medicine. As a group, the major and minor investigators supervise vigorous research programs, share complementary research interests, and are amply supported by NIH mechanisms. The requested instrument will bring together chemists and biomedical scientists in an environment that facilitates the discovery and study of novel small molecules that may ultimately lead to the development of novel therapeutic strategies. As the worldwide genome Initiatives near fruition, we recognize unique opportunities to significantly enhance and extend many of our current studies by incorporation of 96/384-well live-cell based assay strategies. A Molecular Devices Discovery-1 High Content Screening System and associated software and integrated fluidics form the core of this system, which will allow for rapid and systematic quantitative measurements of numerous cell biological events such as neurite outgrowth, cell viability, mitotic rate, mitochondrial dysfunction, receptor clustering, translocation of proteins between cellular compartments, angiogenesis, apoptosis, receptor internalization, exocytosis, cell motility, and others. The requested system will greatly facilitate studies supported by NIH.

描述（由申请人提供）：向国家研究资源中心提出的这项提案寻求资金来建立最先进的高内涵细胞筛选系统，该系统将为埃默里大学医学院生物医学系的研究人员提供服务。作为一个团体，主要和次要研究人员监督积极的研究计划，分享互补的研究兴趣，并得到 NIH 机制的大力支持。所需的仪器将把化学家和生物医学科学家聚集在一个环境中，促进新型小分子的发现和研究，最终可能导致新型治疗策略的开发。随着全球基因组计划即将取得成果，我们认识到通过结合基于 96/384 孔活细胞的检测策略来显着增强和扩展我们当前的许多研究的独特机会。 Molecular Devices Discovery-1 高内涵筛选系统以及相关软件和集成流体学构成了该系统的核心，它将允许快速、系统地定量测量众多细胞生物事件，如神经突生长、细胞活力、有丝分裂率、线粒体功能障碍、受体聚集、细胞区室之间的蛋白质易位、血管生成、细胞凋亡、受体内化、胞吐作用、细胞运动等。所请求的系统将极大地促进 NIH 支持的研究。