JEOL JEM-1011 Transmission Electron Microscope

JEOL JEM-1011 透射电子显微镜

• 批准号: 6877297
• 负责人: Dennis Brown
• 金额: $ 32.14万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877297
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; digital imaging; electron microscopy; transmission electron microscopy

DESCRIPTION (provided by applicant): We request for funds to purchase a JEOL 1011 transmission electron microscope equipped with an AMT digital camera. The new instrument will be housed within the Program in Membrane Biology (PMB) Microscopy Core at the Massachusetts General Hospital (MGH) in the new Charles River Plaza (CRP) research building that will be completed in the summer of 2005. The mandate of this core facility is to provide access to a full range of light and electron microscopy, image analysis, and quantitative methods to individual research groups, 3 PHS funded Centers and 3 new Thematic Centers within the MGH and the Harvard Medical School (HMS) community. These include conventional EM, freeze-fracture EM, immunogold labeling techniques and negative staining. The reasons for this application are to: 1): Provide critically needed instrument time to support increasing demand from current and new users; 2) Provide digital image capability, not previously available but commonly and increasingly requested by users; 3) Replace an existing 17-year old Core microscope that will remain in the "MGH-East" facility when our Microscopy Core moves into the new building, 1.5 miles away. Electron microscopy is an essential service provided by the PMB Core. The current electron microscope used by the Core is a Philips CM10, which is more than 17 years old. It was purchased using Institutional funds in 1986/7. MGH-East is 1.5 miles distant (across the Charles River) from the MGH main campus and the new CRP building. The CM10 will not be moved into the new CRP research building next year and will continue to support the research environment at MGH-East. Other PMB Core equipment that was purchased with PHS and private funds, as well as all PMB scientific faculty and our 3 Core technologists, will move into the new CRP building next year. A new electron microscope is, therefore, essential if the Core is to continue to function to serve the EM needs of its considerable user base at MGH and other Boston area institutions. Since the PMB Core was founded in 1986, it has become a central part of the MGH/HMS medical research community. Over 55 PHS-funded PIs within the HMS/MGH and in other institutions have used the facility in the past 24 months. Access to the EM in particular is now limited by the sheer volume of requests for time. For all of the reasons above, an EM installed in the CRP building is absolutely essential for the Core to continue to provide its services to its existing user base, and at the same time expand its operations to incorporate new users with existing grants, and to help generate new funding.

描述（由申请人提供）： 我们请求资金购买配备 AMT 数码相机的 JEOL 1011 透射电子显微镜。该新仪器将安装在马萨诸塞州总医院 (MGH) 的新查尔斯河广场 (CRP) 研究大楼内的膜生物学 (PMB) 显微镜核心项目中，该大楼将于 2005 年夏季竣工。核心设施是为各个研究小组、麻省总医院和哈佛医学院内的 3 个 PHS 资助中心和 3 个新主题中心提供全方位的光学和电子显微镜、图像分析和定量方法（HMS）社区。这些包括传统电镜、冷冻断裂电镜、免疫金标记技术和负染色。此应用程序的原因是： 1)：提供急需的仪器时间，以支持当前和新用户不断增长的需求； 2) 提供以前不可用但用户普遍且越来越多要求的数字图像功能； 3) 当我们的显微镜核心搬进 1.5 英里外的新大楼时，更换现有的使用了 17 年的核心显微镜，该显微镜仍保留在“MGH-East”设施中。电子显微镜是 PMB Core 提供的一项重要服务。目前Core使用的电子显微镜是Philips CM10，已有17年多的历史了。它是在 1986/7 年使用机构资金购买的。 MGH-East 距离 MGH 主校区和新的 CRP 大楼 1.5 英里（穿过查尔斯河）。明年，CM10 不会搬入新的 CRP 研究大楼，并将继续支持 MGH-East 的研究环境。其他用 PHS 和私人资金购买的 PMB 核心设备，以及所有 PMB 科研人员和我们的 3 名核心技术人员，将于明年搬进新的 CRP 大楼。因此，如果核心要继续发挥作用，满足 MGH 和其他波士顿地区机构的大量用户群的电子显微镜需求，新的电子显微镜就至关重要。自 1986 年 PMB Core 成立以来，它已成为 MGH/HMS 医学研究界的核心部分。在过去 24 个月里，HMS/MGH 和其他机构内超过 55 个由 PHS 资助的 PI 使用了该设施。现在，对 EM 的访问尤其受到大量时间请求的限制。出于上述所有原因，在 CRP 大楼中安装 EM 对于核心继续向其现有用户群提供服务、同时扩大其业务以将新用户纳入现有赠款来说绝对是必要的。帮助筹集新资金。