High Resolution Ultrasound Biomicroscopy System

高分辨率超声生物显微镜系统

• 批准号: 6877272
• 负责人: Daniel Bernstein
• 金额: $ 22.58万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877272
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cardiovascular imaging /visualization

DESCRIPTION (provided by applicant): This is a Shared Instrumentation Grant request for a VisualSonics High Resolution Ultrasound Biomicroscopy System to be shared by a group of established cardiovascular investigators at Stanford University. These six co-investigators have an excellent track record in utilizing transgenic and gene knockout models of human cardiovascular disease to answer basic questions in cardiovascular development, cell signaling, and cardiovascular pathophysiology. Examples of gene targeted animals under current investigation include those with alterations of: a and b-adrenergic receptors, protein kinase C isoenzymes, the apelin/APJ-receptor, microtubule-associated protein light chain 3 (LC3), the calcium binding protein S100A4/Mtsl, and bone morphogenetic protein receptors (BMP-R) I and II. Studies are also underway of transplanted embryonic stem cells and cardiomyocytes overexpressing connexin-43 in murine models of ischemic injury. Models currently being studied include genetic and toxic dilated cardiomyopathy, cardiac hypertrophy, ischemia/reperfusion injury, preconditioning, and cardiac allograft rejection. Echocardiographic assessment of cardiac function is thus critically important to the success of each investigator's research program; currently these assessments are being performed utilizing equipment developed for imaging human neonates. Recent developments in high frequency ultrasound (12.5-49 mHz transducers) have allowed imaging of the adult murine heart with accuracy previously unobtainable and have also allowed imaging of the neonatal and fetal murine heart, providing a major advance for studies of abnormal cardiac development. There is a strong institutional commitment to support the High Resolution Ultrasound System, which will be based in the Stanford Murine Cardiovascular Phenotyping Core, located in the Principal Investigator's lab. This facility has been providing Stanford investigators with physiologic phenotyping services for 10 years: acute and chronic vascular cannulation, telemetry, conductance PV loop analysis, exercise physiology, metabolism, tissue bath studies, and echocardiography. This lab has been at the forefront in developing murine phenotyping techniques, including one of the first to standardize non-restrained, non-anesthetized cardiovascular measurements and to develop murine treadmill exercise protocols. Being an integral part of this core facility will guarantee the quality and reproducibility of High Resolution Ultrasound studies as well as utilize a proven mechanism to provide other qualified Stanford investigators access to this equipment.

描述（由申请人提供）：这是一项共享仪器拨款申请，要求使用 VisualSonics 高分辨率超声生物显微镜系统，由斯坦福大学一组成熟的心血管研究人员共享。这六位共同研究人员在利用人类心血管疾病的转基因和基因敲除模型来回答心血管发育、细胞信号传导和心血管病理生理学的基本问题方面拥有出色的记录。目前正在研究的基因靶向动物的例子包括那些具有以下改变的动物：a和b-肾上腺素能受体、蛋白激酶C同工酶、apelin/APJ-受体、微管相关蛋白轻链3 (LC3)、钙结合蛋白S100A4/ Mtsl 和骨形态发生蛋白受体 (BMP-R) I 和 II。在小鼠缺血性损伤模型中移植的胚胎干细胞和心肌细胞过度表达连接蛋白 43 的研究也在进行中。目前正在研究的模型包括遗传性和中毒性扩张型心肌病、心脏肥大、缺血/再灌注损伤、预处理和心脏同种异体移植排斥。因此，心脏功能的超声心动图评估对于每个研究者的研究计划的成功至关重要；目前，这些评估正在利用为人类新生儿成像开发的设备进行。高频超声（12.5-49 mHz 换能器）的最新发展使得能够以以前无法获得的精度对成年小鼠心脏进行成像，并且还能够对新生儿和胎儿小鼠心脏进行成像，为异常心脏发育的研究提供了重大进展。机构致力于支持高分辨率超声系统，该系统将基于首席研究员实验室的斯坦福小鼠心血管表型分析核心。该设施 10 年来一直为斯坦福大学研究人员提供生理表型分析服务：急性和慢性血管插管、遥测、电导 PV 环路分析、运动生理学、新陈代谢、组织浴研究和超声心动图。该实验室一直处于开发小鼠表型分析技术的最前沿，包括第一个标准化非约束、非麻醉心血管测量和开发小鼠跑步机运动方案的实验室之一。作为该核心设施的一个组成部分，将保证高分辨率超声研究的质量和可重复性，并利用经过验证的机制为其他合格的斯坦福大学研究人员提供使用该设备的机会。