Diagnostic assays for Borrelia Ionestari

爱氏疏螺旋体 (Borrelia Ionestari) 的诊断测定

• 批准号: 7127851
• 负责人: SUSAN E LITTLE
• 金额: $ 7.32万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7127851
• 关键词: Borrelia; Lyme disease; Ungulate; arthropod borne communicable disease; bacterial antigens; borreliosis; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; microorganism classification; microorganism culture; septicemia; serology /serodiagnosis; ticks

DESCRIPTION (provided by applicant): Southern tick-associated rash illness (STARI) is a newly described Lyme disease-like infection reported from the southeastern and south central US caused by Borrelia lonestari. Our long-range goal is to understand the importance of B. lonestari as a human pathogen. The objective of this application is to develop direct microscopic, culture, molecular, and serologic methods that will allow diagnosis of B. lonestari infection in humans, experimental animal models, and reservoir hosts. Borrelia lonestari is most closely related to B. miyamotoi, B. theileri, and the relapsing fever Borrelia, all of which produce high-density spirochetemia during infection. Infection with relapsing fever Borrelia also results in a strong humoral immune response. Our pilot trial infection of two deer fawns showed that B. lonestari also produces a circulating spirochetemia and results in seroconversion of infected animals. The rationale behind the proposed research, therefore, is that like infection with relapsing fever Borrelia spp., B. lonestari infection is likely to result in detectable spirochetemia, allowing culture of the organism from blood, visualization on blood smears, and detection by molecular assays, as well as a strong antibody response which will permit development of immunodiagnostic tools. To accomplish the objectives of this application, we will pursue three specific aims: (1) refine our methods for culturing B. lonestari and for directly identifying spirochetes in circulating blood using samples collected from experimentally infected deer; (2) develop molecular assays for identification of Borrelia lonestari that allow it to be quickly and reliably distinguished from B. burgdorferi and relapsing fever Borrelia; and (3) identify candidate antigens that may elicit production of B. lonestari-specffic antibodies. At the completion of this research, we expect to have developed culture, microscopic, molecular, and serologic techniques that will specifically identify B. lonestari infection. The diagnostic assays resulting from this proposed research will allow accurate identification of the Borrelia spp. causing disease in people and, ultimately, a more robust understanding of the role of B. lonestari as a human pathogen. Improved diagnosis of human B. lonestari infection should result.

描述（由申请人提供）：南部tick相关的皮疹疾病（Stari）是由Borrelia Lonestari引起的美国东南部和中南部报道的新描述的莱姆病样感染。我们的远程目标是了解B. lonestari作为人类病原体的重要性。该应用的目的是开发直接的显微镜，培养，分子和血清学方法，这些方法将允许人类，实验性动物模型和储层宿主诊断出Lonestari感染。 Borrelia lonestari与宫类芽孢杆菌，B。Theileri和复发性发烧硼曲霉最密切相关，它们在感染过程中所有这些都会产生高密度的螺旋血症。感染发烧发烧的疏螺旋体也会导致强烈的体液免疫反应。我们对两只鹿小鹿的试验性试验感染表明，B. lonestari也会产生循环蛋白酶血症，并导致感染动物的血清转化。因此，拟议研究背后的理由是，就像感染一样，发烧发烧疏浆spp。，B。lonestari感染可能会导致可检测的螺旋凝集血症，从而使有机体从血液中培养，从血液上可视化，对血液涂片的可视化以及通过分子测定的检测，并且可以通过强大的抗体反应以及强大的抗体反应来开发免疫能力的工具。为了实现此应用程序的目标，我们将追求三个具体的目标：（1）完善我们培养B. lonestari的方法，并使用从实验感染的鹿中收集的样品直接识别循环血液中的螺旋体； （2）开发用于鉴定疏螺旋体的分子测定法，使其可以快速而可靠地与B. burgdorferi区分开，并散发出发烧的伯氏发烧； （3）确定可能引起lonestari特定抗体的候选抗原。这项研究完成后，我们希望已经开发出培养物，微观，分子和血清学技术，这些技术将专门鉴定出Lonestari的感染。这项提出的研究产生的诊断测定法将允许准确鉴定伯氏菌属。引起人们的疾病，最终对B. lonestari作为人类病原体的作用有更深入的了解。应改善人类芽孢杆菌的诊断。