Overlooked No More: Discovering Pathogens in a National Tick Collection

不再被忽视：在国家蜱虫收藏中发现病原体

基本信息

批准号：
10113526
负责人：
SUSAN E LITTLE
金额：
$ 7.48万
依托单位：
OKLAHOMA STATE UNIVERSITY STILLWATER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-25 至 2022-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10113526
关键词：
Abate Anaplasma phagocytophilum Awareness Babesia Bacterial Infections Biological Assay Biological Models Black-legged Tick Borrelia burgdorferi Canis familiaris Centers for Disease Control and Prevention (U.S.)Collection Complex Country Coupled Data Data Set Diagnosis Disease Ehrlichia Epidemiology Felis catus Future Geographic Distribution Geographic Locations Geography Goals Health Human Infection Knowledge Lyme Disease Maps Molecular North America Outcome Prevalence Public Health Reporting Research Rickettsia Rickettsia Infections Risk Surveillance Program Surveys Tick-Borne Diseases Tick-Borne Infections Ticks Time United States Virus Diseases Work bioinformatics tool combat deep sequencing design disorder risk emerging pathogen evidence base human disease human pathogen innovation metagenome metagenomic sequencing microbiome national surveillance next generation sequencing novel pathogen secondary analysis spotted fever tick population tick-borne tick-borne pathogen vector-borne infection

项目摘要

Project Summary/Abstract Current understanding of the diversity and geographic distribution of tick-borne pathogens in the United States remains limited. The majority of studies reported to date have focused on Borrelia burgdorferi sensu stricto, the causative agent of Lyme disease and the most commonly diagnosed vector-borne infection in the country. However, ticks in the United States also transmit several other pathogens that present additional, significant public health concerns, including spotted fever group Rickettsia spp., Ehrlichia spp., Babesia spp., and Anaplasma phagocytophilum. Moreover, novel viral and bacterial disease agents continue to be described from ticks. Here, we propose a small, self-contained project to perform a secondary analysis of an existing dataset, namely, several thousand ticks collected from more than one thousand dogs and several hundred cats in 49 of the 50 states in 2018 and 2019. Targeted, well- characterized assays will be performed to detect established tick-borne pathogens in the ticks. In addition, we will use next generation sequencing to characterize the metagenome of a subset of the ticks and then query the raw data with e-probes to discover novel agents that may be present. The findings from this work will inform future field efforts to more fully understand the geographic and temporal diversity of tick-borne pathogens in North America; greater understanding of the true public health menace presented by tick-borne infections is expected to result.

项目摘要/摘要当前对tick传播病原体多样性和地理分布的理解美国仍然有限。迄今为止报道的大多数研究都集中在疏螺旋体上 Burgdorferi sensu stricto，莱姆病的病因和最常见的该国诊断出媒介传播的感染。但是，美国的壁虱也传输其他几种病原体，这些病原体提出了其他重要的公共卫生问题，包括斑点发烧组立克属，Ehrlichia spp。，Babesia spp。和Anaplasma 吞噬细胞。此外，继续描述了新型病毒和细菌疾病剂从壁虱。在这里，我们提出了一个小型，独立的项目，以对现有的数据集，即从一千以上收集的数千个壁虱 2018年和2019年50个州中有49只狗和数百只猫。针对性，良好将进行特征测定，以检测壁虱中已建立的tick传播病原体。此外，我们将使用下一代测序来表征子集的元基因组 tick虫，然后与电子探针一起查询原始数据以发现可能是的新型代理展示。这项工作的发现将为未来的现场努力提供信息，以更充分地了解北美壁虱传播病原体的地理和时间多样性；更大对tick传播感染带来的真正公共卫生威胁的理解预计结果。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Identification of Rickettsia spp. and Babesia conradae in Dermacentor spp. Collected from Dogs and Cats Across the United States.

DOI：
10.1089/vbz.2021.0047
发表时间：
2021-12
期刊：
Vector borne and zoonotic diseases (Larchmont, N.Y.)
影响因子：
0
作者：
Duncan KT;Grant A;Johnson B;Sundstrom KD;Saleh MN;Little SE
通讯作者：
Little SE

Ticks infesting dogs and cats in North America: Biology, geographic distribution, and pathogen transmission.

DOI：
10.1016/j.vetpar.2021.109392
发表时间：
2021-06
期刊：
VETERINARY PARASITOLOGY
影响因子：
2.6
作者：
Saleh, Meriam N.;Allen, Kelly E.;Lineberry, Megan W.;Little, Susan E.;Reichard, Mason, V
通讯作者：
Reichard, Mason, V

Geographic Distribution and Seasonality of Brown Dog Tick Lineages in the United States.