Natural variation in Drosophila female postmating traits

果蝇雌性交配后性状的自然变异

• 批准号: 6925655
• 负责人: DAVID J BEGUN
• 金额: $ 26.91万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6925655
• 关键词: Drosophilidae; animal breeding; animal population genetics; arthropod genetics; behavioral /social science research tag; ethology; evolution; female; fertility; gene expression; genetic screening; genotype; male; polymerase chain reaction; quantitative trait loci; reproductive system; sex behavior; sperm analysis

DESCRIPTION (provided by applicant): In flies, as in many other animals, mating does not guarantee successful reproduction. Postmating prefertilization interactions between males and females (e.g., sperm competition, sperm storage by females) also play a central role in reproduction. Moreover, post-copulatory interactions may have broad evolutionary implications. For example, postmating sexual selection may contribute to the evolution of reproductive isolation. Most research on post-copulatory pre-fertilization biology in flies has focused on male-expressed proteins such as seminal fluid proteins. Though it is well known that female flies are not simply passive vessels and that female-mediated interactions with male products are important to reproductive success, little is known about the genetics or population genetics of female postcopulatory biology. The proposed research aims to begin filling this basic gap in our understanding of Drosophila females. The specific aims of the proposed work are to i) use molecular approaches to identify candidate genes potentially affecting female postcopulatory phenotypes in D. melanogaster, ii) use QTL analysis of recombinant inbred lines of D. melanogaster to identify regions of the genome harboring natural variants affecting female post-copulatory phenotypes, iii) use molecular population genetics to test hypotheses on the causes of variation in candidate genes, iv) investigate divergence of female postcopulatory phenotypes and evolution of postcopulatory incompatibilities in the melanogaster group.

描述（由申请人提供）：与许多其他动物一样，在苍蝇中，交配并不能成功地再现。男性和女性之间的终止优先利用相互作用（例如精子竞争，女性的精子储存）在生殖中也起着核心作用。此外，填充后相互作用可能具有广泛的进化意义。例如，延迟性选择可能有助于生殖隔离的演变。大多数关于果蝇后吞噬前生物学生物学的研究都集中在雄性表达的蛋白质（例如精确液蛋白）上。尽管众所周知，雌性苍蝇不仅是被动血管，而且与雄性产品的相互作用对于生殖成功很重要，但对女性后生物学的遗传学或种群遗传学知之甚少。拟议的研究旨在开始在我们对果蝇女性的理解中填补这一基本差距。 拟议工作的具体目的是i）使用分子方法来鉴定d。belanogaster中潜在影响女性的基因，潜在地影响女性后孔子后表型，ii）使用QTL分析。候选基因的变异，iv）研究女性后表型的差异以及Melanogaster组中腔后不相容性的演变。