Natural variation in Drosophila female postmating traits

果蝇雌性交配后性状的自然变异

• 批准号: 7033001
• 负责人: DAVID J BEGUN
• 金额: $ 26.4万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7033001
• 关键词: Drosophilidae; animal breeding; animal population genetics; arthropod genetics; behavioral /social science research tag; ethology; evolution; female; fertility; gene expression; genetic screening; genotype; male; polymerase chain reaction; quantitative trait loci; reproductive system; sex behavior; sperm analysis

DESCRIPTION (provided by applicant): In flies, as in many other animals, mating does not guarantee successful reproduction. Postmating prefertilization interactions between males and females (e.g., sperm competition, sperm storage by females) also play a central role in reproduction. Moreover, post-copulatory interactions may have broad evolutionary implications. For example, postmating sexual selection may contribute to the evolution of reproductive isolation. Most research on post-copulatory pre-fertilization biology in flies has focused on male-expressed proteins such as seminal fluid proteins. Though it is well known that female flies are not simply passive vessels and that female-mediated interactions with male products are important to reproductive success, little is known about the genetics or population genetics of female postcopulatory biology. The proposed research aims to begin filling this basic gap in our understanding of Drosophila females. The specific aims of the proposed work are to i) use molecular approaches to identify candidate genes potentially affecting female postcopulatory phenotypes in D. melanogaster, ii) use QTL analysis of recombinant inbred lines of D. melanogaster to identify regions of the genome harboring natural variants affecting female post-copulatory phenotypes, iii) use molecular population genetics to test hypotheses on the causes of variation in candidate genes, iv) investigate divergence of female postcopulatory phenotypes and evolution of postcopulatory incompatibilities in the melanogaster group.

描述（由申请人提供）：与许多其他动物一样，苍蝇的交配并不能保证成功繁殖。雄性和雌性之间的交配后受精前相互作用（例如精子竞争、雌性储存精子）也在繁殖中发挥着核心作用。此外，交配后相互作用可能具有广泛的进化意义。例如，交配后性选择可能有助于生殖隔离的进化。大多数关于果蝇交配后受精前生物学的研究都集中在雄性表达的蛋白质上，例如精液蛋白质。尽管众所周知，雌性果蝇不仅仅是被动的血管，而且雌性介导的与雄性产品的相互作用对于繁殖成功很重要，但人们对雌性交配后生物学的遗传学或群体遗传学知之甚少。拟议的研究旨在填补我们对雌性果蝇理解中的这一基本空白。 拟议工作的具体目标是 i) 使用分子方法来识别可能影响黑腹果蝇雌性交配后表型的候选基因，ii) 使用黑腹果蝇重组自交系的 QTL 分析来识别含有自然变异的基因组区域影响雌性交配后表型，iii) 使用分子群体遗传学来检验候选基因变异原因的假设，iv) 研究雌性交配后表型的差异黑腹果蝇群体交配后不相容性的表型和进化。