Diagnosis of Ovarian Cancer by Confocal Microendoscopy

共聚焦显微内窥镜诊断卵巢癌

• 批准号: 6958231
• 负责人: ARTHUR F. GMITRO
• 金额: $ 28.98万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6958231
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; clinical biomedical equipment; clinical research; confocal scanning microscopy; contrast media; diagnosis design /evaluation; early diagnosis; endoscopy; female; fluorescence microscopy; fluorescent dye /probe; human subject; intravital microscopy; laboratory mouse; neoplasm /cancer diagnosis; ovary neoplasms; patient oriented research

DESCRIPTION (provided by applicant): The objective of this research is to develop a screening tool for the early detection of ovarian cancer with the capability of diagnosing microscopic cancers or areas of abnormality in the ovary using minimally invasive surgical access. Ovarian cancer has the highest mortality of any of the gynecologic conditions because it is usually diagnosed in late stages leading to poor overall prognosis for survival. The fluorescence confocal microendoscope is a new type of instrument for visualizing tissue at the cellular level that has shown great promise for performing optical biopsy. Preliminary work suggests significant potential for this instrument in imaging and identifying pathology in the ovary. Confocal microscopy has the capability to enhance a physicians' diagnostic ability and ultimately provide an automated image analytic tool for real-time in vivo diagnosis of pathology. The specific aims of the work are as follows: 1) To build a third-generation confocal microendoscope instrument that can be used routinely and reliably in the clinic. The system will be compact, mobile, and incorporate high sensitivity grayscale and multi-spectral imaging capabilities. 2) To develop a reliable mechanism for localized delivery of exogenous fluorescence contrast agent. Ideally this will be a non- (or minimum) contact technique that will allow well-controlled exposure of tissue to the exogenous agents. 3) To incorporate automated real-time pattern recognition capability into the system. This capability will assist the operating physician in identifying pathology during continuous imaging of the surface of the ovary. 4) To evaluate the safety and efficacy of specific fluorescent compound(s) for use as contrast agents in the peritoneal cavity that will be delivered topically to the surface of the ovary. This work will be done in a rodent model. 5) To demonstrate the feasibility of using the instrument for in vivo imaging of human ovary. This will prove that the basic system concept works and to point out practical issues that need to be addressed. 6) To assess the efficacy of the clinical instrument for detection of pathology of the ovary. This will be a more extensive study to evaluate the sensitivity and specificity of this technique across a range of histologic diagnoses including normal ovary, benign neoplasms, and cancers. The clinical evaluations will be done in a cohort of women who are scheduled to undergo oophrectomy procedures.

描述（由申请人提供）： 这项研究的目的是开发一种筛查工具，用于早期检测卵巢癌，并使用微创手术诊断卵巢中的微观癌症或异常区域进行诊断。卵巢癌在任何妇科疾病中的死亡率最高，因为它通常在晚期被诊断出，导致生存的总体预后不佳。荧光共聚焦微型内注射镜是一种在细胞水平上可视化组织的新型仪器，它显示出对进行光学活检的巨大希望。初步工作表明，该仪器在成像和识别卵巢病理学方面的重要潜力。共聚焦显微镜具有增强医师诊断能力的能力，并最终提供了一种自动化的图像分析工具，用于实时体内诊断病理学。该作品的具体目的如下：1）建造可以在诊所常规可靠地使用的第三代共焦微型内脏仪器。该系统将紧凑，移动，并结合高灵敏度灰度和多光谱成像功能。 2）开发一种可靠的机制，用于局部递送外源荧光对比剂。理想情况下，这将是一种非（或最小）接触技术，它将允许组织对外源性剂的暴露良好。 3）将自动实时模式识别能力纳入系统。该能力将有助于手术医师在卵巢表面连续成像期间鉴定病理学。 4）评估特异性荧光化合物（S）的安全性和功效，以用作腹膜腔中的对比剂，并将其局部递送到卵巢表面。这项工作将在啮齿动物模型中完成。 5）证明使用该仪器进行体内卵巢成像的可行性。这将证明基本系统概念有效并指出需要解决的实际问题。 6）评估临床仪器检测卵巢病理学的功效。这将是一项更广泛的研究，以评估该技术在包括正常卵巢，良性肿瘤和癌症在内的一系列组织学诊断中的敏感性和特异性。临床评估将在计划进行卵子切除术程序的一群女性中进行。