CORE--ANIMAL PHYSIOLOGY AND GENE TRANSFER FACILITY

核心——动物生理学和基因转移设施

• 批准号: 7098693
• 负责人: JOHN ROSS
• 金额: $ 20.76万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7098693
• 关键词: angiocardiography; bioimaging /biomedical imaging; biomedical facility; blood flow measurement; disease /disorder model; echocardiography; heart function; heart imaging /visualization /scanning; intracardiac pressure; laboratory mouse; microsurgery; myocardial infarction; phenotype; transfection; ventricular hypertrophy

DESCRIPTION (provided by applicant): This core unit will be available to all investigators on the Program Project and will provide physiologic and morphologic characterization of cardiovascular phenotypes, using miniaturized hemodynamic and imaging methods along with microsurgical techniques for in vivo physiological studies in mice. The goals of this core facility are to: 1) provide detailed phenotyping of genetically engineered murine models using imaging to assess functional cardiac morphology, primarily transthoracic echocardiography and contrast angiocardiography, and a variety of hemodynamic techniques for evaluating the physiologic status of the heart; 2) apply microsurgical methods in mice for inducing pressure overload on either the left or right ventricle, or to produce myocardial infarction, in order to induce ventricular remodeling with hypertrophy or heart failure; and 3) extend to mice high efficiency in vivo cardiac gene transfer methods recently developed in hamsters and rats.

描述（由申请人提供）： 该核心单元将适用于该计划项目的所有调查员，并将提供 心血管表型的生理和形态表征，使用 微型血流动力学和成像方法以及体内的显微外科技术 小鼠的生理研究。 该核心设施的目标是：1）提供基因工程的详细表型 使用成像评估功能性心脏形态的鼠模型，主要是经胸膜 超声心动图和对比度血管造影以及各种血流动力学 评估心脏生理状态的技术； 2）在 小鼠在左室或右心室诱导压力超负荷，或产生 心肌梗塞，以诱导肥大或心脏的心室重塑 失败; 3）最近扩展到小鼠的体内心脏基因转移方法高效率 在仓鼠和大鼠中发育。