Biomedical National Elemental Imaging Resource (BNEIR)

生物医学国家元素成像资源 (BNEIR)

• 批准号: 10614502
• 负责人: Brian P Jackson
• 金额: $ 46.42万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10614502
• 关键词: Ablation; Acceleration; Aluminum; Alzheimer' s Disease; Antibodies; Area; Arsenicals; Basic Science; Biochemical; Biological Markers; Biological Process; Biology; Brain; Cell Cycle Regulation; Cell physiology; Cells; Centers of Research Excellence; Chemicals; Coenzymes; Collaborations; Communities; Computer software; Consult; Contrast Media; Copper; Custom; Cytometry; Data Analyses; Detection; Diagnosis; Disease; Doctor of Philosophy; Drug Delivery Systems; Drug Targeting; E-learning; Elements; Ensure; Fees; Fluorescence; Fostering; Foundations; Funding; Gadolinium; Goals; Health; Hepatolenticular Degeneration; Homeostasis; Human; Human Resources; Human body; Image; Imaging Techniques; Immunity; Immunohistochemistry; Inductively Coupled Plasma Mass Spectrometry; Infrastructure; Institution; International; Internet; Iron; Isotopes; Knowledge; Laboratories; Lasers; Life; Lifting; Light; Link; Location; Magnetic Resonance Imaging; Metals; Mission; Molecular; National Institute of General Medical Sciences; Online Systems; Organ; Organism; Performance; Persons; Platinum Compounds; Prevention; Process; Proteins; Reproduction; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Roentgen Rays; Role; Science; Scientist; Selenium; Senile Plaques; Services; Source; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Spottings; Synchrotrons; System; Techniques; Technology; Therapeutic; Time; Tissues; Trace Elements; Training; Training Programs; United States National Institutes of Health; Vacuum; Visit; Work; Zinc; base; beamline; bioimaging; biological systems; biomedical facility; biomedical resource; cancer therapy; cofactor; college; data acquisition; data handling; detection limit; diagnostic tool; disease diagnosis; drug efficacy; experience; experimental study; human disease; improved; industry partner; innovation; instrumentation; mass spectrometer; medical schools; meetings; metalloenzyme; nanoparticle; programs; repaired; response; software development; success; synchrotron radiation; webinar

Up to 60 chemical elements can be detected in the human body, about 25 of which are essential to life. Elements such as iron (Fe), zinc (Zn), copper (Cu), and selenium (Se) are constituents of enzyme cofactors required for a wide range of processes from cell cycle regulation, reproduction, structural repair, and immunity. Dysregulation of these elements is a hallmark of disease. For instance, aluminum (Al), Fe, Cu and Zn accumulation in amyloid plaques in the brain are associated with Alzheimer’s disease and dysregulation of Cu causes Menkes and Wilson’s disease. Chemical elements are also diagnostic tools and chemotherapeutics; gadolinium (Gd) is the most common magnetic resonance imaging contrast agent, metal-base nanoparticles show promise for targeted drug delivery and platinum (Pt) and arsenic (As) compounds are used in cancer treatment. Invariably, knowing the spatial distribution of an element in an organ, tissue or cell is essential to fully investigate mechanisms of disease or the efficacy of drug delivery. Hence, elemental imaging at the micron scale is essential to further the NIGMS goal to support basic research that increases our understanding of biological processes and lays the foundation for advances in disease diagnosis, treatment, and prevention. Current analytical resources for biomedical elemental imaging are either over-subscribed (synchrotron X-ray fluorescence), cover only a limited range of elements, are performed under a vacuum or not readily scalable to imaging at the mm scale. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a laboratory-based elemental imaging (elemental imaging) technique that it is increasingly being applied to biomedical applications with comparable or lower detection limits than other elemental imaging techniques and, potentially, easier access and operability. To date there are no elemental imaging LA-ICP-MS user resources in the US. The Dartmouth Trace Element Analysis Core is an established and highly regarded shared resource that supports researchers throughout the US, is staffed by experienced researchers in elemental imaging and ICP-MS, uses state-of-the-art instrumentation and has excellent links to industry partners. Our aims are to expand upon our current ad hoc elemental imaging service to establish an elemental imaging user facility; the Biomedical National Elemental Imaging Resource (BNEIR), which will accelerate and simplify access for biomedical researchers to instrumentation, expertise, web-based and in-person training, after-visit support, software and will foster a dynamic community for elemental imaging users. We will actively promote the elemental imaging within the larger NIGMS community of scientists through webinars, seminars, attendance at national and international meetings and actively seeking out NIH researchers currently pursuing relevant research projects.

在人体中可以检测到多达60个化学元素，其中大约25个对生命至关重要。诸如铁（Fe），锌（Zn），铜（Cu）和硒（SE）之类的元素是从细胞周期调节，繁殖，结构修复和免疫学所需的广泛过程所需的酶辅因子的结构。这些元素的失调是疾病的标志。例如，大脑中淀粉样蛋白斑块中的铝（AL），Fe，Cu和Zn积累与阿尔茨海默氏病有关，CU的失调会导致Menkes和Wilson病。化学元素也是诊断工具和化学治疗药。 Gadolinium（GD）是最常见的磁共振成像对比剂，金属碱纳米颗粒显示出靶向药物递送和铂（PT）和砷（AS）化合物的希望。知道器官，组织或细胞中元素的空间分布总是至关重要的，对于充分研究疾病机制或药物递送的有效性至关重要。因此，在微米量表上进行的元素成像对于进一步支持基础研究的基础研究至关重要，从而增加了我们对生物过程的理解，并为疾病诊断，治疗和预防的进步奠定了基础。当前用于生物医学元素成像的分析资源要么超出订阅（同步子X射线荧光），仅覆盖有限的元素，在真空下进行，或者在MM尺度上不容易扩展到成像。激光消融电感耦合的等离子体质谱法（LA-ICP-MS）是一种基于实验室的元素成像（元素成像）技术，它越来越多地应用于具有可比或较低检测限制的生物医学应用中，而不是其他元素成像技术，并且具有潜在的，更容易访问和操作。迄今为止，美国没有元素成像LA-ICP-MS用户资源。达特茅斯痕量元素分析核心是一种已建立且备受推崇的共享资源，支持整个美国的研究人员，由经验丰富的元素成像和ICP-MS的研究人员组成，使用了最先进的仪器，并与行业合作伙伴有良好的联系。我们的目的是扩展我们当前的临时元素成像服务，以建立元素成像用户设施；生物医学国家元素成像资源（Bneir）将加速并简化生物医学研究人员的仪器，专业知识，基于Web的和个人培训的访问权限，访问后的支持，软件，并将促进一个为元素成像用户提供动态的社区。我们将通过网络研讨会，精确参加国家和国际会议，积极地促进更大的科学家社区中的元素成像，并积极寻找目前从事相关研究项目的NIH研究人员。