Trace Elements (TE)

微量元素 (TE)

• 批准号: 10554268
• 负责人: Brian P Jackson
• 金额: $ 2.11万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-04 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10554268
• 关键词: Animal Organ; Arsenic; Beverages; Biological; Blood; Budgets; Cancer Center; Cancer Center Support Grant; Carcinogens; Catchment Area; Cells; Cereals; Chemicals; Chromatography; Coupled; Data; Elements; Ensure; Exposure to; Fishes; Funding; Hair; Health Policy; Human; Individual; Inductively Coupled Plasma Mass Spectrometry; Infant; Inorganic Chemistry; Institution; International; Invertebrates; Iron; Link; Malignant Neoplasms; Measures; Methods; Micronutrients; Monitor; NCI Center for Cancer Research; Norris Cotton Cancer Center; Placenta; Platinum; Play; Population Sciences; Preparation; Protocols documentation; Public Health; Quality Control; Research; Research Personnel; Resource Sharing; Resources; Role; Running; Sampling; Selenium; Services; Soil; Structure of nail of toe; Technology; Testing; Therapeutic Effect; Therapeutic Uses; Tissues; Trace Elements; Urine; Water; Work; analytical method; animal tissue; bioimaging; case-by-case basis; early life exposure; granite; instrument; instrumentation; member; nanoparticle; programs; quality assurance

Project Summary/Abstract: Trace Elements The chemistry of inorganic elements is linked inextricably with the study of cancer. This is especially true for our catchment area, given the high level of inorganic element exposures typical of “the Granite State”. Whether studying known carcinogens, such as arsenic, the potential antagonistic effects of elements, such as selenium, or the therapeutic uses of platinum or iron nanoparticles, there is a clear need for both routine and advanced trace element analytical methods to support NCCC research. The Trace Elements (TE) Shared Resource provides access to low-level inorganic elemental analysis to all four research programs within the Cancer Center. TE uses state-of-the-art ICP-MS instruments to quantify multiple elements and, when coupled with chromatography, multiple contaminant compounds (species) in a variety of biological and environmental media. Cutting-edge ICP-MS instrumentation (e.g., triple-quadrupole ICP-QQQ) provides rapid, interference- free analysis of established and emerging contaminants and macro- and micro-nutrient elements. The breadth of sample types handled by TE spans water, soil, sediment, invertebrates, fish and animal tissues and organs, cell lysates, human hair, toenail, blood, urine, placenta, foodstuffs, and beverages. The ability to handle such a broad array of sample types is a unique feature of TE. In some cases, the sample mass available for analysis is <10 mg, and methods often are adapted to account for small sample masses on a case-by-case basis. Analytical data must be collected according to accepted protocols for sample preparation, analysis, and quality control. TE has established protocols and a Quality Assurance program, uses methods based on those of the CDC, FDA, EPA, and USGS; and participates in national and international proficiency testing programs to ensure accurate, precise, and defensible data. TE has very strong links to the Cancer Population Sciences (CPS) Program. TE is an internationally-recognized center for low-level analysis of chemical elements and their compounds in biological tissues. TE has provided services for 27 other US academic institutions over the past funding cycle and fulfills a unique niche in providing state-of-the-art analytical services in trace elemental analysis. Over the last funding cycle, TE has run 6,721 samples for 12 NCCC Members from all 4 NCCC Programs (CPS [1], CBT [1], ICI [2], and TEC [8]). Funded NCCC Members have represented 15% (10) of Total Users, and we are requesting only 3% of the Total TE Budget from CCSG support. One focus of the CPS Program is early life exposure to arsenic, and TE is integral to the determination and speciation of arsenic for this project. This work has directly informed state and national public health policy. For example, the US FDA announced a proposed limit for inorganic arsenic in infant cereal of 100 ppb in 2016. For the TEC Program, TE provided a method for measuring iron nanoparticles in animal tissue. TE has contributed to CBT investigators monitoring the therapeutic effects of platinum. TE is demonstrably an essential, efficient and high-impact resource that continues to play a pivotal role in NCCC research.

项目摘要/摘要：微量元素 无机元素的化学与癌症的研究无关紧要。尤其如此 鉴于典型的“花岗岩状态”的无机元素暴露水平高，我们的集水区域。 研究已知的致癌物，例如砷，元素的潜在拮抗作用，例如硒， 或铂或铁纳米颗粒的治疗用途，显然需要常规和高级 痕量元素分析方法支持NCCC研究。跟踪元素（TE）共享资源 为癌症中的所有四个研究计划提供了低水平无机元素分析的访问权限 中心。 TE使用最先进的ICP-MS仪器来量化多个元素，并且与 色谱法，多种生物学和环境中的多种污染物（物种） 媒体。尖端的ICP-MS仪器（例如，三肢ICP-QQQ）提供快速，干扰 - 免费分析已建立和新兴的污染物以及宏观和微营养元素。广度 由水，土壤，沉积物，无脊椎动物，鱼类和动物组织和器官处理的样品类型 细胞裂解物，人头发，脚趾甲，血液，尿液，胎盘，食物和卧室。处理这样的能力 广泛的样本类型是TE的独特功能。在某些情况下，样品质量可用于分析 IS <10 mg，方法通常会根据具体情况来考虑小样本质量。 必须根据公认的方案收集分析数据，以进行样品制备，分析和质量 控制。 TE已经建立了协议和质量保证计划，使用了基于该协议的方法 CDC，FDA，EPA和USGS；并参加国家和国际能力测试计划 确保准确，精确和可辩护的数据。 TE与癌症人群科学有很强的联系 （CPS）程序。 TE是一个国际公认的化学元素低水平分析和 它们在生物组织中的化合物。 TE为其他27个美国学术机构提供了服务 过去的资金周期并实现了在痕量元素中提供最先进的分析服务时独特的利基市场 分析。在上一个融资周期中，TE从所有4个NCCC中为12名NCCC成员运行了6,721个样品 程序（CPS [1]，CBT [1]，ICI [2]和TEC [8]）。资助的NCCC成员代表15％（10） 总用户，我们仅要求CCSG支持的TE总预算总额的3％。 CPS的一个焦点 计划是早期寿命暴露于砷中，TE是砷的确定和规范不可或缺的一部分 这个项目。这项工作直接了解了州和国家公共卫生政策。例如，美国FDA 宣布了2016年婴儿谷物100 ppb的无机砷的拟议限制。对于TEC计划，TE TE 提供了一种测量动物组织中铁纳米颗粒的方法。 TE为CBT调查人员做出了贡献 监测铂的治疗作用。 TE显然是必不可少的，高效的和高影响的 在NCCC研究中继续发挥关键作用的资源。