Laser Ablation-ICPTOF for fast multi-element imaging of biomedical samples

用于生物医学样品快速多元素成像的激光烧蚀-ICPTOF

• 批准号: 10427847
• 负责人: Brian P Jackson
• 金额: $ 66.28万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10427847
• 关键词: Ablation; Arts; Biological; Cells; Communities; Coupled; Data; Elements; Fostering; Frequencies; Funding; Image; Imaging Techniques; Inductively Coupled Plasma Mass Spectrometry; Knowledge; Laboratories; Lasers; Link; Measurable; Monoclonal Antibody R24; National Institute of General Medical Sciences; Organ; Physiologic pulse; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scanning; Signal Transduction; Spatial Distribution; Specimen; Spottings; Switzerland; System; Time; Tissues; Trace Elements; United States National Institutes of Health; Width; base; biomedical imaging; data acquisition; design; detection limit; disease diagnosis; experience; imaging facilities; industry partner; instrumentation; millisecond; model design; programs; success

Knowledge of the spatial distribution of elements within cells, organs and tissue can help inform mechanisms of disease, diagnosis, and treatment. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a laboratory-based elemental imaging technique that it is increasingly being applied to biomedical applications with comparable or lower detection limits than other elemental imaging techniques and, potentially, easier access and operability. The Dartmouth Trace Element Analysis Core (TEAC) is an established and highly regarded shared resource that supports researchers throughout the US, is staffed by experienced researchers in elemental imaging and ICP-MS and has excellent links to industry partners. In 2021 the TEAC was funded by NIGMS (R24 GM141194-01) to establish the Biomedical National Elemental Imaging Resource (BNEIR) to accelerate and simplify access for biomedical researchers to instrumentation, expertise, and to foster a dynamic community for elemental imaging users. To truly make BNIER a world class elemental Imaging facility we proposed to upgrade the current TEAC LA-ICP-MS instrumentation to a faster laser ablation system with an ultrafast washout cell (imageBIO266, Elemental Scientific Lasers, Bozeman, MT) and a time-of-flight ICP-MS (ICPTOF). The imageBIO266 is newly designed specifically for imaging biological specimens with a laser frequency of up to 1000 Hz as opposed to the 20Hz of our current laser, meaning that it can scan (image) up to 50 times faster than our current system. The fast wash out sample cell (TV3) of this new system delivers the laser pulse to the ICP-MS with pulse widths of 1 -3 msec and thus provides exceptional signal to background ratios. However, only ICPTOF systems have fast enough data acquisition to collect per pixel data at the 50 – 1000HZ laser firing rates and short pulse widths. Within the last five years these fast laser ablation-ICPTOF systems have become the state for the art for fast elemental imaging. The icpTOF S2 (TOFWERK, Switzerland) is a high sensitivity time-of-flight ICP-MS model designed specifically for applications such as biomedical elemental imaging. The icpTOF S2 collects all possible elemental data every 30 µsec, whereas conventional quadrupole ICP-MS (Q-ICP-MS) systems are much slower (msec), especially when multiple elements are collected. By contrast, the ImageBIO266 coupled to an icpTOF S2 can image 10 – 50X faster and by default, collects all measurable elements. The highest resolution (smallest laser spot size) of the ImageBIO266 is 1 µm and collecting data at this cellular-level resolution would only be practical from a time standpoint with an icpTOF S2. Hence, the full potential of higher resolution, faster laser firing frequency, and faster washout sample cell of the imageBIO266 can only be fully utilized by icpTOF S2. This cutting-edge instrumentation will be pivotal in the success of our NIGMS-funded elemental imaging resource and will immediately benefit our NIH-funded users, including regional INBRE programs in IDeA states, in the quality and spatial resolution of their data and increased sample throughput.

了解细胞内元素的空间分布，器官和组织可以帮助告知机制 疾病，诊断和治疗。激光消融电感耦合等离子体质谱法（LA-ICP-MS） 是一种基于实验室的元素成像技术，它越来越多地应用于生物医学应用 与其他元素成像技术相比，具有可比或较低的检测极限，并且可能更容易访问 和运营。达特茅斯痕量元素分析核心（TEAC）是一个已建立且高度考虑的 共享资源，支持整个美国的研究人员，由经验丰富的研究人员组成 元素成像和ICP-MS，与行业合作伙伴有良好的联系。 2021年，TEAC由 NIGMS（R24 GM141194-01）建立生物医学国家元素成像资源（Bneir） 加速并简化生物医学研究人员访问仪器，专业知识并促进动态 元素成像用户的社区。真正使Bnier成为世界一流的元素成像设施我们 提议将当前的TEAC LA-ICP-MS仪器升级为更快的激光消融系统 超快冲洗单元（ImageBio266，元素科学激光器，Bozeman，MT）和飞行时间ICP-MS （ICPTOF）。 ImageBio266是专门设计用于用激光对生物标本进行成像的 与当前激光的20Hz相反，最多1000 Hz的频率，这意味着它可以扫描（图像）至 比我们当前系统快50倍。该新系统的快速洗涤样品单元格（TV3）提供了 激光脉冲至ICP -MS，脉冲宽度为1-3毫秒，因此为背景提供了非凡的信号 比率。但是，只有ICPTOF系统具有足够快的数据获取，可以在50 - 1000Hz激光射击速率和短脉冲宽度。在过去的五年内，这些快速激光消融 - icptof 系统已成为快速元素成像的艺术状态。 S2（瑞士TOFWERK）ICPTOF 是专为生物医学等应用而设计的高灵敏度ICP-MS模型 元素成像。 S2 ICPTOF每30 µsec收集所有可能的元素数据，而常规 四极核ICP-MS（Q-ICP-MS）系统慢得多（MSEC），尤其是当多个元素是 集。相比之下，ImageBio266耦合到iCptof S2可以快速10 - 50倍，默认情况下， 收集所有可测量的元素。 ImageBio266的最高分辨率（最小的激光斑点尺寸）为1 µm 并且在此蜂窝级分辨率上收集数据只有从iCptof的时间角度就可以实用。 S2。因此，更高分辨率，更快的激光射击频率和更快的冲洗样品单元的全部潜力 ImageBio266只能通过ICPTOF S2充分利用。这种尖端的仪器将在 我们的NIGMS资助的元素成像资源的成功，将立即使我们的NIH资助用户受益 包括区域近亲计划在思想状态中，以其数据的质量和空间分辨率以及增加 样品吞吐量。