REACTION MECHANISM & EFFICIENCY IN OSCILLATORY REACTIO

反应机制

• 批准号: 3298934
• 负责人: JOHN ROSS
• 金额: $ 11万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3298934
• 关键词: bioenergetics; capillary; chemical kinetics; chemical reaction; electrical potential; electron transport; enzyme mechanism; gel electrophoresis; horseradish peroxidase; light intensity; mass spectrometry; molecular dynamics; nicotinamide adenine dinucleotide; oscillography; oxidation; photosynthesis; photosystem; stoichiometry; thermodynamics; ultraviolet spectrometry

The purpose of this research is to apply new methods of determining reaction mechanisms Of oscillatory biological reactions. Photosynthesis in plants (C3) and the horseradish peroxidase reactions are suggested for detailed study by measuring the temporal response of each system to a series of external perturbations (shift experiments, destabilization experiments, phase determinations). These measurements lead to partial experimental determination of the Jacobian of the non-linear kinetics of each system, and from there to a classification based on a method of categorization of oscillatory systems proposed previously. The experimental methods to be used for detection of chemical species in both types of systems include fluorescence detection, specific electrode measurements, mass spectrometric detection of gaseous species, and capillary zone electrophoresis. The last analytic technique allows temporal measurements of concentrations of far larger number of species than previously attempted. Further studies are planned on the efficiency of these reactions, particularly for the photosynthesis system; and on the dependency of efficiency on an oscillatory intensity of light input as well as the variation in efficiency with changes in the average intensity and the frequency of the variation of the light intensity. A model of oscillatory photosynthetic reactions will be constructed; no satisfactory model is available to date. Finally, the connection will be attempted between specific non-linearity in each system and possible changes in efficiency with external variations of constraints. Oscillatory biological reactions are ubiquitous in living systems and their reaction mechanisms must be understood in relation to control, and proper or diseased functioning, as for example in mitochondria or endocrine systems.

这项研究的目的是应用确定的新方法 振荡生物反应的反应机制。 光合作用 建议提出植物（C3）和辣根过氧化物酶反应 通过测量每个系统对A的时间响应来详细研究 一系列外部扰动（班次实验，不稳定 实验，相位确定）。 这些测量导致部分 非线性动力学的雅各布的实验测定 每个系统，从那里到那里的分类 先前提出的振荡系统的分类。 这 实验方法用于检测两者的化学物种 系统类型包括荧光检测，特定电极 测量，气态物种的质谱检测以及 毛细管区电泳。 最后的分析技术允许 浓度的时间测量远大 比以前尝试过。 计划进一步研究效率 在这些反应中，特别是对于光合系统；在 效率对光输入的振荡强度的依赖性 随着效率的变化，平均强度的变化和 光强度变化的频率。 一个模型 将构建振荡的光合作用反应；不满意 型号可供迄今为止使用。 最后，将尝试连接 在每个系统中的特定非线性之间以及可能的变化 效率与约束的外部变化。 振荡生物学 反应在生命系统及其反应机制中无处不在 必须了解控制，适当或患病 功能，例如在线粒体或内分泌系统中。