THERMODYNAMIC EFFICIENCY OF BIOLOGICAL PUMPS

生物泵的热力学效率

• 批准号: 3298930
• 负责人: JOHN ROSS
• 金额: $ 9.41万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3298930
• 关键词: 6 phosphofructokinase; adenosine triphosphate; bioenergetics; biological signal transduction; chromatophore; fructose phosphate; photochemistry; thermodynamics

An experimental study is proposed on the thermodynamic efficiency of biochemical and biological energy transduction devices (pumps). The specific system proposed consists of the glycolytic reaction of fructose- 6-phosphate with ATP to produce fructose-diphosphate and ADP, the reaction being catalyzed by the enzyme phosphofructokinase. This reaction, in a vessel, is driven by the illumination of added chromatophores which regenerate ATP. The reverse reaction is catalyzed in a second vessel connected to the first by a membrane. The membrane is permeable to all chemical species except enzymes. Upon illumination of the first vessel a gradient in concentrations of FDP and F6P is established between the two vessels and this gradient can be related to the efficiency of the pumping process, which is the ratio of the power output divided by the power input. Measurements are planned on the efficiency for periodically varying light intensity at various frequencies and amplitudes compared to the efficiency with steady illumination. Prior theories predict the possibility of increasing the efficiency of such nonlinear pumps by means of external periodic variations of constraints such as the illumination, and the purpose of the measurements is to test these predictions. A second system is the proton pump in bacteriorhodopsin, either by itself or coupled to catalyzed by horseradish peroxidase (HPO). The HPO reaction is known to be oscillatory. An understanding of the thermodynamic efficiency of nonlinear energy transduction systems is important for the foundation of bioenergetics necessary for investigation of related diseases such as myopathies (genetic mitochondrial diseases).

提出了一项有关热力学效率的实验研究 生化和生物学转导设备（泵）。 这 提出的特定系统由果糖的糖酵解反应组成 6-磷酸ATP产生果糖 - 二磷酸盐和ADP，反应 被酶磷酸激酶酶催化。 这个反应，在 容器是由添加色谱的照明驱动的 再生ATP。 反应在第二容器中催化 由膜连接到第一个。 膜可渗透到所有人 除酶以外的化学物种。 第一艘船只A照明时 两者之间建立了FDP和F6P浓度的梯度 容器和该梯度可能与泵送的效率有关 过程，即功率输出的比率除以功率输入。 计划在效率上进行定期变化的效率测量 与效率相比，各种频率和振幅的强度 具有稳定的照明。 先前的理论预测了 通过外部提高此类非线性泵的效率 诸如照明等约束的周期性变化和 测量的目的是测试这些预测。 第二个系统 是细菌紫红质中的质子泵，还是本身或耦合 由辣根过氧化物酶（HPO）催化。 已知HPO反应是 振荡。 了解非线性的热力学效率 能源转导系统对于基础很重要 研究相关疾病（例如 肌病（遗传线粒体疾病）。