CORE--ANIMAL PHYSIOLOGY FACILITY

核心——动物生理设施

• 批准号: 6242126
• 负责人: JOHN ROSS
• 金额: $ 27.08万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6242126
• 关键词: biomedical facility; disease /disorder model; laboratory mouse; microsurgery; physiology; ventricular hypertrophy

The mouse has become the preferred species for genetic manipulation, but approaches for characterizing the mouse cardiac phenotype and performing physiologic studies on cardiovascular function on the intact mouse heart in vivo generally have lagged behind the rapid advances in transgenic and gene-deletion technologies. This mouse physiology core will improve and apply miniaturized hemodynamic and imaging techniques for physiological studies, in intact adult mice, as well as in intact mouse embryos. Also, techniques for studying isolated cardiomyocytes will be available, along with an approach for viral transfection of the heart. Microsurgical techniques also have been devised in mice to produce chronic pressure overload on either the left and right ventricle, as well as chronic volume overload. Miniaturized imaging techniques for the heart involve transthoracic ultrasound (2-D, M-mode and Doppler echocardiography) and x-ray microangiography for adult mouse hearts, and intravital videomicroscopy for embryonic mouse hearts. High- fidelity catheter tip micromanometry is now available for assessing intracardiac pressures and myocardial contractility in anesthetized open or closed-chest animals, as well as in the unanesthetized state.

小鼠已成为基因操纵的首选物种， 但是用于表征小鼠心脏表型的方法和 对完整的心血管功能进行生理研究 鼠标心脏在体内通常落后于快速进步 在转基因和基因缺失技术中。 这种小鼠生理 核心将改善并应用微型血流动力学和成像 生理研究的技术，完整的成年小鼠以及 完整的小鼠胚胎。 此外，用于研究孤立的技术 将提供心肌细胞，并提供病毒疗法的方法 心脏转染。 在小鼠中也设计了微外科技术以产生 左室和右心室的慢性压力超负荷也 作为慢性体积超负荷。 微型成像技术 心脏涉及经胸超声（2-D，M模式和多普勒 超声心动图）和成年小鼠心脏的X射线显微镜图， 以及用于胚胎小鼠心脏的静脉内镜检查。 高的- 富达导管尖端微观管理现在可以评估 麻醉的心脏内压力和心肌收缩性 开放式或闭合的动物，以及未经麻醉的状态。