Glutamate Biosensors for Ischemia or Single-Cell Studies

用于缺血或单细胞研究的谷氨酸生物传感器

• 批准号: 6833691
• 负责人: DAVID A JOHNSON
• 金额: $ 9.81万
• 依托单位: PINNACLE TECHNOLOGY, INC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-20 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833691
• 关键词: aminoacid oxidase; artificial membranes; bioengineering /biomedical engineering; biomedical equipment development; biosensor device; biotechnology; clinical biomedical equipment; computer data analysis; computer program /software; computer system design /evaluation; diffusion; electrical measurement; enzyme activity; enzyme substrate complex; glutamates; immobilized enzymes; ischemia; miniature biomedical equipment; neurotoxicology; neurotoxins; single cell analysis; time resolved data

DESCRIPTION (provided by applicant): Pinnacle Technology and Dr. George Wilson of the University of Kansas will design a sensor suite capable of measuring physiologically relevant levels of glutamate, in vivo, in untethered small animals, with high spatial resolution and under ischemic conditions. Research has shown that the major cause of cell death following an ischemic stroke is not hypoxia, but radically elevated levels of glutamate (glutamate excitotoxicity). The ischemic event has been well characterized in terms of blood flow and tissue oxygenation, but other than microdialysis, there is no tool currently available to directly measure glutamate excitotoxicity. Microdialysis is of limited use due to its poor temporal resolution. Electrochemical sensors work well for electroactive species, but not for non-electroactive species. Behavioral scientists also seek to make connections between the brain and behavior. For these studies high spatial resolution is needed. In Phase I, needle-type biosensors (180 mu/m diameter) will be developed to detect large concentrations of glutamate during ischemic conditions. High spatial resolution biosensors (76 mu/m diameter), will also be developed for normal oxygen conditions ( >40 torr). In Phase II, the team will develop a 76 mu/m ischemic glutamate sensor and 10 um glutamate sensors for single-cell measurements and associated electronics.

描述（由申请人提供）：Pinnacle Technology 和堪萨斯大学的 George Wilson 博士将设计一套传感器套件，能够在缺血条件下以高空间分辨率测量不受束缚的小动物体内谷氨酸的生理相关水平。研究表明，缺血性中风后细胞死亡的主要原因不是缺氧，而是谷氨酸水平急剧升高（谷氨酸兴奋性毒性）。缺血事件已在血流和组织氧合方面得到了很好的表征，但除了微透析之外，目前没有可直接测量谷氨酸兴奋毒性的工具。微透析由于时间分辨率差而用途有限。电化学传感器适用于电活性物质，但不适用于非电活性物质。行为科学家还试图在大脑和行为之间建立联系。这些研究需要高空间分辨率。在第一阶段，将开发针型生物传感器（直径180μ/m）来检测缺血条件下高浓度的谷氨酸。 高空间分辨率生物传感器（直径 76 μ/m）也将针对正常氧气条件（>40 托）而开发。在第二阶段，该团队将开发用于单细胞测量和相关电子设备的 76 μ/m 缺血性谷氨酸传感器和 10 um 谷氨酸传感器。

项目成果

In vivo biosensors.

体内生物传感器。

• DOI: 10.1111/j.1742-4658.2007.06077.x
• 发表时间: 2007
• 期刊: The FEBS journal
• 作者: Wilson,GeorgeS;Ammam,Malika
• 通讯作者: Ammam,Malika

In-vivo electrochemistry: What can we learn about living systems?

• DOI: 10.1021/cr068082i
• 发表时间: 2008-07-01
• 期刊: CHEMICAL REVIEWS
• 影响因子: 62.1
• 作者: Wilson, George S.;Johnson, Michael A.
• 通讯作者: Johnson, Michael A.