Functionalized Multi-Modal Tetrode Arrays for Real-Time, Site-Specific Neurochemical Monitoring

用于实时、特定部位神经化学监测的功能化多模态 Tetrode 阵列

• 批准号: 10759908
• 负责人: DAVID A JOHNSON
• 金额: $ 28.98万
• 依托单位: PINNACLE TECHNOLOGY, INC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10759908
• 关键词: Active Sites; Adenosine; Adult; Animals; BRAIN initiative; Behavior; Behavioral; Biogenic Amines; Biosensing Techniques; Biosensor; Blood capillaries; Bolus Infusion; Brain; Catalogs; Cellular Structures; Charge; Chemicals; Choline; Cognitive; Computer software; Conscious; Data Collection; Deposition; Development; Diameter; Dopamine; Electrodes; Electronics; Electrophysiology (science); Elements; Ensure; Environment; Enzymes; Glass; Glucose; Glutamates; Goals; Hand; Hour; Individual; Kansas; Life; Light; Location; Maps; Measurement; Measures; Mechanics; Mental disorders; Modality; Monitor; Neurosciences; Neurotransmitters; North Carolina; Oxidases; Oxygen; Pattern; Peer Review; Performance; Periodicity; Phase; Platinum; Psyche structure; Publications; Reporting; Reproducibility; Research Personnel; Rodent; Sales; Scanning; Serine; Serotonin; Site; Slice; Software Design; Stimulus; Strategic Planning; Symptoms; Synapses; System; Techniques; Technology; Time; United States; United States National Institutes of Health; Universities; Work; ascorbate; carbon fiber; cell type; commercialization; data quality; design; economic cost; experience; experimental study; flexibility; glucose oxidase; in vivo; insight; instrumentation; interest; meter; multimodality; neural; neural patterning; neurochemistry; novel; optogenetics; photoactivation; prototype; response; sensor; symposium; temporal measurement; tool; wireless; wireless electronic

ABSTRACT The goal of this proposal is to develop a multimodal, multianalyte sensor system including wired and wireless electronics and software. This new suite of tools will enable simultaneous recording of amperometric sensors (glutamate, glucose, lactate, choline, and/or oxygen), electrophysiology, and fast-scan cyclic voltammetry (biogenic amines) in conscious, freely moving rodents. The proposed array and measurement system will enable the synchronous measurement of chemical and electrical conditions in the rodent brain. This suite will include new instrumentation and software for simultaneous, synchronized data collection and a novel functionalized tetrode array that will enable the recording of multiple modalities and analytes at a single location in the brain. An ideal multi-modal sensor would support a wide variety of measurements in a minimal volume, with no chemical or electrical crosstalk and without altering the local environment of the sensor, while allowing long- term, quantifiable, high spatial and temporal resolution, measurements of only the analytes/phenomena of interest. In practice, all measurement modalities are limited, but by combining multiple modalities in a single, configurable probe, researchers can leverage the strengths of the individual techniques to best suit the experiment at hand. The Brain-2025 Report (https://braininitiative.nih.gov/strategic-planning/brain-2025-report) states: “Our charge is to understand the circuits and patterns of neural activity that give rise to mental experience and behavior. To achieve this goal for any circuit requires an integrated view of its component cell types, their local and long‐range synaptic connections, their electrical and chemical activity over time, and the functional consequences of that activity at the levels of circuits, the brain, and behavior. Combining these elements is at present immensely difficult even for one circuit, yet we must also weave together the many interlocking circuits in a single brain. As the President said in his White House press conference, this is indeed a “grand challenge for the 21st century.” The proposed functionalized tetrode array, instrumentation, and support software will give researchers the experimental flexibility required to pursue these goals while ensuring experimental consistency (mechanical design), data quality (electronics design), experimental flexibility (software design), maximizing overall reproducibility while minimizing rodent use.

抽象的 该提案的目的是开发多模式的多模型传感器系统，包括有线和无线 电子和软件。这套新的工具将使您可以简单地记录安培传感器 （谷氨酸，谷氨酸，乳糖，胆碱和/或氧），电生理学和快速扫描的环状伏安法 （生物胺）在有意识的自由移动啮齿动物中。拟议的阵列和测量系统将启用 啮齿动物大脑中化学和电条件的同步测量。这个套房将包括 最简单，同步的数据收集和新型功能化的新仪器和软件 Tetrode阵列将在大脑中的一个位置记录多种方式和分析物。 理想的多模式传感器将支持最少的各种测量，没有 化学或电串扰，而无需改变传感器的本地环境，同时允许长长 术语，可量化，高空间和临时分辨率，仅测量分析物/现象 兴趣。实际上，所有测量方式都有限，但是通过将多种方式结合在一起 可配置的探测器，研究人员可以利用单个技术的优势来最适合 手头的实验。 Brain-2025报告（https://braininitiative.nih.gov/strategic-planning/brain-2025-report） 国家： “我们的指控是了解神经元活动的电路和模式，这会导致心理经验和 行为。为了实现任何电路的目标，需要对其组件单元类型的集成视图，其本地 和远程突触连接，随着时间的推移其电和化学活性以及功能 该活动在电路，大脑和行为水平上的后果。结合这些元素是 即使在一个电路中也非常困难，但是我们还必须将许多互锁电路编织在一起 在一个大脑中。正如总统在他的白宫新闻发布会上所说的那样，这确实是一个“大挑战” 在21世纪。” 拟议的功能化四极阵列，仪器和支持软件将为研究人员提供 实验灵活性才能实现这些目标，同时确保实验一致性（机械 设计），数据质量（电子设计），实验灵活性（软件设计），总体上最大化 可重现性，同时最大程度地减少啮齿动物的使用。