A turn-key optogenetics and electrophysiology measurement system

交钥匙光遗传学和电生理学测量系统

• 批准号: 8979335
• 负责人: DAVID A JOHNSON
• 金额: $ 46.75万
• 依托单位: PINNACLE TECHNOLOGY, INC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8979335
• 关键词: Aging; Animals; Area; Astrocytes; Behavior; Behavioral; Biosensor; Brain; Brain region; Cannulas; Cell Culture Techniques; Cells; Chemistry; Computer software; Data; Development; Development Plans; Devices; Disease; Electroencephalography; Electromyography; Electronics; Electrophysiology (science); Elements; Equipment; Event; Feedback; Fiber; Fiber Optics; Genetic; Goals; Head; Heart Diseases; Hospitalization; Individual; Joints; Kansas; Knowledge; Laboratories; Lasers; Light; Longevity; Malignant Neoplasms; Measurement; Mechanics; Medical center; Mental disorders; Methods; Mus; Neuraxis; Neurons; Neurosciences; Neurotransmitters; New York; Operative Surgical Procedures; Opsin; Optical Methods; Optics; Oranges; Output; Pharmacologic Substance; Phase; Physiology; Premature Mortality; Process; Progress Reports; Protocols documentation; Rattus; Research; Research Personnel; Rodent; Signal Transduction; Single Seizures; Solutions; Source; Stimulus; System; Systems Analysis; Techniques; Time; United States; Universities; Validation; Visual; Wireless Technology; Work; base; behavior measurement; burden of illness; commercialization; cost; design; digital; experience; improved; in vivo; multidisciplinary; optogenetics; product development; prototype; public health relevance; research study; severe mental illness; skills; software systems; success

 DESCRIPTION (provided by applicant): The goal of this project is to design and commercialize tethered and wireless turn-key optogenetics and electrophysiological/neurotransmitter/behavior measurement systems for use in mice and rats. Optogenetics harnesses a combination of genetic and optical methods to directly control neuronal events in specific cells of the central nervous system. These methods are broadly applicable, but can be specifically used to provide an unprecedented understanding of cortical activity and aging. The optogenetics field is maturing and there are numerous commercial sources for optogenetic components; however, the technique requires a multidisciplinary skill set including chemistry, optics, physiology, electronics, mechanics, software, and systems analysis. To date, any single experiment requires a system designed from individual, component parts. Many researchers also have existing equipment that they desire to incorporate into a full optogenetics system. This may include lasers, cameras and potentially behavioral hardware and software platforms. In these situations, a digital timing protocol (TTL) is often used to maintain synchronization, but there are subtleties (device latency, etc.) to this approach that are often overlooked. The proposed system will be capable of delivering multiple, selectable wavelengths of light to one or more specific brain regions while simultaneously recording electrical signals, neurotransmitters and behavior in rodents throughout the lifespan of the animal. All synchronization between the electrophysiological, mechanical and visual inputs, and optical and stimulus outputs will be precisely controlled via a master timing, digital input/output platform as well as sophisticated software timing techniques. The LED fiber probe connects via a simple electrical connection to a headstage. This removes the need for fiber optic rotary joints and enables precise control of the amount of light delivered. When completed, this system will significantly improve scientific knowledge by providing a turn-key solution for researchers from multiple fields to seamlessly integrate optogenetic control alongside traditional pharmaceutical, aging and other studies.

 描述（由适用提供）：该项目的目的是设计和商业化束缚和无线交钥匙光遗传学以及用于小鼠和大鼠使用的电生理/神经递质/行为测量系统。光遗传学利用了遗传和光学方法的组合，可以直接控制中枢神经系统特定细胞中的神经元事件。这些方法广泛适用，但可专门用于提供对皮质活动和衰老的前所未有的理解。光遗传学领域正在成熟，并且有许多用于光遗传学成分的商业来源。但是，该技术需要一个多学科技能集，包括化学，光学，生理，电子，力学，软件和系统分析。迄今为止，任何单个实验都需要由单个组件零件设计的系统。许多研究人员还拥有他们希望将其纳入完整的光遗传学系统的现有设备。这可能包括激光器，相机以及潜在的行为硬件和软件平台。在这些情况下，数字计时协议（TTL）通常用于维持同步，但是这种方法通常会忽略这种方法（设备延迟等）。所提出的系统将能够在同时记录的同时，将多个可选的光的光波长传递到一个或多个特定的大脑区域。整个动物生命周期中的啮齿动物的电信号，神经递质和行为。电生理，机械和视觉输入以及光学和刺激输出之间的所有同步都将通过主正时，数字输入/输出平台以及软软件时正时技术来精确控制。 LED纤维探针通过简单的电气连接连接到媒体。这消除了对光纤旋转接头的需求，并可以精确控制传递的光量。完成后，该系统将通过为从多个领域的研究人员提供旋转解决方案来显着改善科学知识，从而无缝将光遗传学控制与传统的药物，衰老和其他研究一起。