Commercialization of Enzyme Modified Carbon-Fiber Electrodes Paired with Voltammetry for Simultaneous Real-Time Monitoring of Electroactive and Non-Electroactive Species at Discrete Brain Locations

酶改性碳纤维电极与伏安法相结合的商业化，用于同时实时监测离散大脑位置的电活性和非电活性物质

• 批准号: 9903459
• 负责人: DAVID A JOHNSON
• 金额: $ 44.5万
• 依托单位: PINNACLE TECHNOLOGY, INC
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9903459
• 关键词: Acetylcholine; Adult; BRAIN initiative; Behavioral Research; Benchmarking; Biogenic Amines; Biosensor; Brain; Caliber; Calibration; Chemicals; Chemistry; Choline; Communities; Complex; Computer software; Couples; Custom; Data; Data Analyses; Detection; Development; Dopamine; Electrodes; Endocrine; Enzymes; Glucose; Glutamates; Goals; Hydrogen Peroxide; Intuition; Laboratories; Learning; Life; Location; Marriage; Measurement; Measures; Mediating; Mental disorders; Methods; Microelectrodes; Monitor; Motivation; Neuropeptides; Neurosciences; Neurotransmitters; North Carolina; Operative Surgical Procedures; Oxidation-Reduction; Performance; Periodicity; Peroxides; Pharmacotherapy; Phase; Physiological; Physiological Processes; Procedures; Production; Protocols documentation; Reporting; Research; Research Personnel; Resolution; Role; Scanning; Signal Transduction; Site; Small Business Innovation Research Grant; Symptoms; System; Techniques; Technology; Time; Training; Translating; United States; United States National Institutes of Health; Universities; Validation; base; brain research; carbon fiber; clinical application; commercialization; data acquisition; design; drug development; economic cost; glucose oxidase; glucose sensor; in vivo; innovation; insight; interest; nervous system disorder; neurochemistry; neurotransmission; pre-clinical; rapid detection; real time monitoring; sensor; sensor technology; software development; technology development; temporal measurement; tool; web site

ABSTRACT Commercially available biosensors are designed to measure only one molecule at a time at a given recording site. This is a problem because chemical signals in the brain do not work in isolation; rather, neurotransmission involves many chemical species simultaneously released and little is known about how specific neurochemicals fluctuate relative to one another. Understanding these relationships is critical to the development of drugs and treatments for a wide range of neurological disorders. The Sombers Lab has established the feasibility of using fast-scan cyclic voltammetry (FSCV) and carbon-fiber microelectrodes for the simultaneous detection of rapid dopamine fluctuations and those of non-electroactive species, such as glucose, at the same recording site. This is done with higher spatial and temporal resolution than currently available methods. The goal of this Lab to Marketplace: Tools for Brain and Behavioral Research SBIR is to translate and commercialize the technology developed by the Sombers team at North Carolina State University. The first goal is to transfer the core technology for the co-detection of dopamine and glucose from the Sombers laboratory to Pinnacle Technology, a company that has developed, manufactured and sold biosensors and electrochemical measurement systems worldwide. Pinnacle-produced sensors will be fully characterized and detailed specifications for the technology (sensitivity, linear range, shelf-life and benchmarks for in vivo performance) will be outlined. The second goal is to develop training tools and software to minimize the learning curve associated with the proper implementation, characterization and analysis of FSCV in research or pre-clinical applications. This will be accomplished by modifying existing Pinnacle software to create an intuitive platform for acquisition and analysis of voltammetry data using the commercial probes. Finally, high production value training videos will be created and made freely available on the Pinnacle website. These will detail experimental procedures for all aspects of in vivo voltammetry including probe calibration, surgical procedures, and data acquisition and analysis protocols. Overall, this project is innovative, because it departs from the status quo by utilizing the redox activity inherent to enzymatically generated H2O2 to identify targeted non-electroactive species, even in the presence of electroactive molecules that are typically excluded as interferents. It is significant, because it combines two state-of-the-art and well-characterized technologies for neurochemical monitoring in a clever, straightforward, and unprecedented manner to provide the community with an established tool that can be used to study the role of glucose in complex physiological processes ranging from basic endocrine function to motivation. It promises to have a transformative effect on neuroscience by allowing researchers interested in diverse aspects of brain function to better understand how these specific neurochemicals rapidly co-fluctuate in discrete brain locations.

抽象的 市售的生物传感器旨在一次在给定记录下一次测量一个分子 地点。这是一个问题，因为大脑中的化学信号不能孤立起作用。而是神经传递 涉及许多同时释放的化学物种，并且对特定的特定方式知之甚少 神经化学物质相对于彼此而波动。了解这些关系对 开发多种神经系统疾病的药物和治疗方法。 Sombers实验室有 确定了使用快速扫描环状伏安法（FSCV）和碳纤维微电极的可行性 同时检测快速多巴胺的波动和非电动活性物种的观察，例如 葡萄糖，在同一记录地点。这是通过比当前更高的空间和时间分辨率完成的 可用方法。该实验室到市场的目标：大脑和行为研究工具SBIR是 翻译和商业化北卡罗来纳州Sombers Team开发的技术 大学。第一个目标是转移核心技术，以从 开发，制造和销售的公司的Sombers Pinnacle Technology 全球生物传感器和电化学测量系统。顶峰生产的传感器将完全 该技术的特征和详细规范（灵敏度，线性范围，保质期和基准测试 对于体内性能）将概述。第二个目标是开发培训工具和软件以最大程度地减少 与适当的实施，表征和分析FSCV相关的学习曲线 研究或临床前应用。这将通过将现有的Pinnacle软件修改为 创建一个直观平台，用于使用商业探针获取和分析伏安法数据。 最后，将创建高生产价值培训视频，并在顶峰上自由使用 网站。这些将详细详细介绍体内伏安法的各个方面的实验程序，包括探针 校准，手术程序以及数据采集和分析方案。总体而言，这个项目是创新的， 因为它通过利用酶生成的H2O2固有的氧化还原活动来偏离现状 识别靶向的非电动活性物种，即使在存在通常是电活性分子的情况下 被排除为干扰物。这很重要，因为它结合了两个最先进且特征良好的 以巧妙，直接且前所未有的方式提供神经化学监测的技术 具有既定工具的社区，可用于研究葡萄糖在复杂生理中的作用 从基本的内分泌功能到动机的过程。它有望对 神经科学通过使研究人员对大脑功能的各个方面感兴趣，以更好地了解 这些特定的神经化学物质在离散的大脑位置迅速脱离。