Perfluoropolymers as New Matrixes for Clinical Sensors

全氟聚合物作为临床传感器的新基质

• 批准号: 6904356
• 负责人: PHILIPPE PJ BUHLMANN
• 金额: $ 21.87万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6904356
• 关键词: animal tissue; artificial membranes; atomic force microscopy; bioengineering /biomedical engineering; biotechnology; blood tests; chemical synthesis; chloride ion; clinical chemistry; electrochemistry; electrolytes; fluosol; human tissue; hydrogen ions; lipids; polyethylene glycols; polymers; potentiometry; proteins; receptor; solubility; spectrometry; urinalysis

DESCRIPTION (provided by applicant): Chemical sensors based on receptor-doped polymeric membranes were developed for over 60 analytes. They are routinely used worldwide in clinical chemistry for well over a billion measurements per year. Unfortunately, their lifetimes are limited by lipids and hydrophobic proteins that adsorb onto or are extracted into these membranes. This hinders their wider use, in particular for the long-term implantation into the human body. Perfluorocarbon matrixes have great promise to solve this problem of sensor lifetime because their low polarity limits the solubility of lipids and proteins. The extraordinarily low polarity of perfluorocarbons is illustrated by the following example: On the pi * scale of solvent polarity, water has a pi* value of 1, hexane defines 0, and perfluorooctane has the astounding value of-0.41. Indeed, it is well documented that lipids are poorly soluble in perfluorocarbons. Therefore, it is expected that nonpolar perfluorinated membrane matrixes will not lose their selectivies when exposed to biological fluids containing lipids and hydrophobic proteins. Moreover, nonpolar perfluorinated matrixes (i) will exhibit much higher selectivities than conventional receptor-doped sensor matrixes because lipophilic interferents are hardly solvated in perfluorinated phases, (ii) are chemically very inert, and (iii) were shown to promote cell growth on their surface to a much lesser extent than most polymers presently used for receptor-based sensors. Despite the attractiveness of perfluorinated phases, the current literature does not describe any receptorbased sensor with a nonpolar perfluorinated matrix. Neither receptors nor electrolyte salts that are soluble in perfluorocarbon phases were reported. This research will develop perfluorinated polymeric matrixes and fluorophilic ion exchanger sites required for the preparation of receptor-based sensors. Three representative fluorophilic receptors will be synthesized and used for the preparation of potentiometric sensors. The robustness of these sensors will be tested with pure solutions of individual lipids, blood serum and urine samples. In brief, specific goals include the development of (i) fluorophilic cations and anions suitable as ionic sites for potentiometric sensors, (ii) a fluorophilic electrolyte salt, (iii) perfluorinated polymers suitable as matrixes for potentiometric sensors, (iv) proton-, creatininium-, and chloride-selective sensors based on perfluorinated membranes for applications in biological samples, and (v) perfluorinated sensing membranes surface-modified with poly(ethylene glycol) for a further improvement of their biocompatibility. The introduction of perfluorinated sensor matrixes will enhance the selectivity, robustness and lifetime of chemical sensors for clinical chemistry. It will also permit other novel uses of receptor-based chemical sensors under harsh conditions, such as long-term environmental monitoring with sensor networks for the prediction of earthquakes and process control in the food industry and manufacturing.

描述（由申请人提供）：为60多个分析物开发了基于受体掺杂聚合物膜的化学传感器。它们通常在全球范围内用于临床化学，每年超过十亿次测量。不幸的是，它们的寿命受到吸附或提取到这些膜上的脂质和疏水蛋白的限制。这阻碍了他们更广泛的用途，特别是用于长期植入人体。全氟化合物基质有很大的希望可以解决这个传感器寿命的问题，因为它们的极性低限制了脂质和蛋白质的溶解度。以下示例说明了全氟化合物的极低极性：在溶剂极性的PI *尺度上，水的PI *值为1，己烷定义0，而PertluorooCtane的惊人值为-0.41。确实，有充分的文献证明，脂质可溶于全氟化合物。因此，预计在暴露于含有脂质和疏水蛋白的生物液时，非极性全氟化膜基质不会失去其选择性。此外，非极性全氟化矩阵（i）的选择性比传统的受体掺杂传感器矩阵表现高得多，因为在全氟化相的相比，几乎不溶解了溶剂化，（ii）在化学上非常惰性，并且（III）比大多数Polymers的表面上的细胞在其表面上促进了细胞的生长。 尽管全氟化相具有吸引力，但目前的文献并未描述任何具有非极性全氟基质的受体传感器。均未据报道全氟化合物相的受体和电解质盐。这项研究将开发全氟化的聚合物基质和制备基于受体的传感器所需的荧光离子交换器位点。将合成三个代表性的荧光受体，并用于制备电位测量传感器。这些传感器的鲁棒性将通过单个脂质，血清和尿液样品的纯溶液进行测试。 In brief, specific goals include the development of (i) fluorophilic cations and anions suitable as ionic sites for potentiometric sensors, (ii) a fluorophilic electrolyte salt, (iii) perfluorinated polymers suitable as matrixes for potentiometric sensors, (iv) proton-, creatininium-, and chloride-selective sensors based on perfluorinated membranes for applications in生物样品和（v）通过聚乙二醇（乙二醇）表面修饰的全氟化传感膜，以进一步改善其生物相容性。 全氟传感器矩阵的引入将提高化学传感器的临床化学传感器的选择性，鲁棒性和寿命。它还将允许在恶劣条件下对基于受体的化学传感器的其他新颖用途，例如使用传感器网络进行长期环境监测，以预测食品工业和制造业中的地震和过程控制。