PHybrid Quadrupole/Orthogonal Time-of-Flight Mass Spec

PHybrid 四极杆/正交飞行时间质谱

• 批准号: 6441003
• 负责人: KYM Francis FAULL
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441003
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; mass spectrometry; matrix assisted laser desorption ionization; proteomics

DESCRIPTION (provided by applicant): Funds are requested to purchase a hybrid quadrupole-time-of-flight laser mass spectrometer (QTOF) for the UCLA Pasarow Mass Spectrometry Laboratory. This is a shared facility jointly supported by the Department of Chemistry and Biochemistry (College of Letters and Sciences) and the Department of Psychiatry and Biobehavioral Sciences (School of Medicine) that is available to the entire UCLA campus research and teaching enterprise. There is no instrument of this type on the UCLA campus. Because of their sensitivity and selectivity, so-called QTOF instruments have been proven to be indispensable for the rapid and reliable identification of proteins from a variety of sources including spots excised from polyacrylamide gel electrophoretograms. This instrument will be a central feature of the UCLA proteomics initiative which is now being established. An instrument of QTOF design with all accessory components necessary for routine protein identification, is essential for the continued productivity, success and competitiveness of a growing number of campus research programs. Reflecting this diversity of use, descriptions of the research of the major users of the instrument are provided.

描述（由申请人提供）：需要资金购买混合动力车 用于 UCLA Pasarow 的四极飞行时间激光质谱仪 (QTOF) 质谱实验室。这是由以下机构共同支持的共享设施 化学与生物化学系（文理学院） 以及精神病学和生物行为科学系（学院） 医学）可供整个加州大学洛杉矶分校校园研究和教学 企业。加州大学洛杉矶分校校园内没有此类仪器。由于 所谓的 QTOF 仪器的灵敏度和选择性已得到证实 对于快速可靠地鉴定蛋白质是必不可少的 多种来源，包括从聚丙烯酰胺凝胶上切下的斑点 电泳图。该仪器将成为加州大学洛杉矶分校的核心特征 目前正在建立蛋白质组学倡议。 QTOF 仪器 设计包含常规蛋白质所需的所有辅助成分 识别，对于持续的生产力、成功和 越来越多的校园研究项目的竞争力。反思 这种用途的多样性，主要用户的研究描述 提供仪器。