Nano-HPLC-ESI Quadrupole Ion Trap Mass Spectrometer

Nano-HPLC-ESI 四极杆离子阱质谱仪

• 批准号: 6876889
• 负责人: Alan V. Smrcka
• 金额: $ 34.04万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2006-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876889
• 关键词: biological signal transduction; biomedical equipment purchase; electrospray ionization mass spectrometry; microarray technology; protein sequence; proteomics

DESCRIPTION (provided by applicant): We request funds for the purchase of an electrospray-ionization ion trap mass spectrometer for protein and proteome analysis. The specific instrument is a Thermo Finnigan LTQ linear quadrupole ion trap mass spectrometry system coupled to a Surveyor HPLC system capable of 100 nL to uL flow rates and a nano or electrospray source. No instrument with comparable capabilities is available at the University of Rochester. There is a need for this instrument as demonstrated by eight major users and at least two minor users, all of whom are funded by NIH. Alan V. Smrcka (Principal Investigator) has been using hydrogen/deuterium exchange MALDI-TOF mass spectrometry for analysis of signal transduction proteins. Acquiring this instrumentation would greatly advance the level of this analysis. Other major users propose to use the system to study global protein modifications in yeast (E. Phizicky), proteins involved in nuclear transport of histones (J. Hayes), analysis of transcription factor interactions in Drosophila (D. Bohmann), proteins involved in histone transport (J .Hayes), proteomic analysis ofneurodegenerative diseases (D. Pearce), Posttranslational modifications of G protein coupled receptors (P. Hinkle), comprehensive analysis of the human salivary proteome (J. Melvin), and tubulin and histone modifications in Tetrahymena (M. Gorovsky). The LC/ESI ion trap mass spectrometer will be incorporated into a functional genomics core facility that also contains a MALDI-TOF and microarray instrumentation. A Masters level mass spectrometrist will be responsible for the day to day operation of the instrument. A Mass Spectrometry Committee has been named, that includes an outside advisory member who directs a mass spectrometry core facility at Vanderbilt University that will provide long-term guidance about the use of the instrument. The institutional commitment will provide salary support for the Masters level mass spectrometrist for three (3) years and will pay the service contract for three (3) years. A cost recovery plan will be implemented that will cover costs in future years.

描述（由申请人提供）：我们请求资金购买用于蛋白质和蛋白质组分析的电喷雾电离离子阱质谱仪。具体仪器是 Thermo Finnigan LTQ 线性四极杆离子阱质谱系统，与流量为 100 nL 至 uL 的 Surveyor HPLC 系统以及纳米或电喷雾源相结合。罗切斯特大学没有具有类似功能的仪器。八个主要用户和至少两个次要用户证明了对该工具的需求，所有这些用户均由 NIH 资助。 Alan V. Smrcka（首席研究员）一直在使用氢/氘交换 MALDI-TOF 质谱法来分析信号转导蛋白。获得这种仪器将大大提高这种分析的水平。其他主要用户建议使用该系统研究酵母中的整体蛋白质修饰（E. Phizicky）、参与组蛋白核运输的蛋白质（J. Hayes）、分析果蝇中的转录因子相互作用（D. Bohmann）、参与组蛋白核转运的蛋白质（D. Bohmann）、组蛋白转运 (J.Hayes)、神经退行性疾病的蛋白质组学分析 (D. Pearce)、G 蛋白偶联受体的翻译后修饰 (P. Hinkle)、人类的综合分析唾液蛋白质组（J. Melvin），以及四膜虫中的微管蛋白和组蛋白修饰（M. Gorovsky）。 LC/ESI 离子阱质谱仪将被纳入功能基因组学核心设施中，该设施还包含 MALDI-TOF 和微阵列仪器。硕士级质谱师将负责仪器的日常操作。已经任命了一个质谱委员会，其中包括一名外部顾问成员，负责指导范德比尔特大学的质谱核心设施，该设施将提供有关仪器使用的长期指导。该机构承诺将为硕士级质谱师提供三 (3) 年的薪资支持，并将支付三 (3) 年的服务合同费用。将实施成本回收计划，以覆盖未来几年的成本。