4000 Q TRAP triple quadrupole/linear ion trap LC/MS/MS

4000 Q TRAP 三重四极杆/线性离子阱 LC/MS/MS

• 批准号: 6880765
• 负责人: Bradford Wayne Gibson
• 金额: $ 50万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6880765
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; high performance liquid chromatography; liquid chromatography mass spectrometry; oxidative stress; phosphorylation; posttranslational modifications

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an Applied Biosystems 4000 Q-TRAP HPLC-MS triple quadrupole linear ion-trap mass spectrometer with nano-electrospray ionization source. This integrated platform will include a Dionex on-line nano-capillary HPLC system with autosampler and switching device for sample enrichment. The Q-TRAP mass spectrometer will be equipped with a Turbo V Source, a TurbolonSpray probe, a NanoSpray Source and Interface. A full complement of instrument software required for data acquisition and data processing is also requested to support the research aims described in this proposal. In brief, we propose to operate this instrument almost exclusively (>95% use) for on-line nano HPLC-MS/MS for the separation and analysis of complex protein/peptide mixtures. These analyses will emphasize the high sensitivity and special scan features of the 4000 Q TRAP, including multiple reaction monitoring, and precursor ion and neutral loss scanning functions. These unique capabilities will be directed at the identification and quantitation of a wide array of biologically important enzymatic and non-enzymatic posttranslational modifications, including protein phosphorylation, oxidative damage and nitrosylation. The primary, but not exclusive focus, will be on supporting a wide array of NIH-funded projects at the Buck Institute that seek to understand the molecular basis for aging and age-related diseases as they correlate to changes in biologically relevant posttranslational modifications.

描述（由申请人提供）：此 NCRR 共享仪器提案寻求资金购买具有纳米电喷雾电离源的 Applied Biosystems 4000 Q-TRAP HPLC-MS 三重四极杆线性离子阱质谱仪。该集成平台将包括 Dionex 在线纳米毛细管 HPLC 系统，配有自动进样器和用于样品富集的切换装置。 Q-TRAP 质谱仪将配备 Turbo V 源、TurbolonSpray 探头、NanoSpray 源和接口。还需要数据采集和数据处理所需的全套仪器软件来支持本提案中描述的研究目标。简而言之，我们建议几乎专门（>95% 使用）操作该仪器用于在线纳米 HPLC-MS/MS，以分离和分析复杂的蛋白质/肽混合物。这些分析将强调 4000 Q TRAP 的高灵敏度和特殊扫描功能，包括多反应监测以及母离子和中性丢失扫描功能。这些独特的功能将针对广泛的生物学上重要的酶促和非酶促翻译后修饰的鉴定和定量，包括蛋白质磷酸化、氧化损伤和亚硝基化。主要但不是唯一的重点将是支持巴克研究所的一系列由美国国立卫生研究院资助的项目，这些项目旨在了解衰老和年龄相关疾病的分子基础，因为它们与生物学相关的翻译后修饰的变化相关。

项目成果

Quantitative mapping of reversible mitochondrial Complex I cysteine oxidation in a Parkinson disease mouse model.

帕金森病小鼠模型中可逆线粒体复合物 I 半胱氨酸氧化的定量图谱。

• 发表时间: 2011-03-04
• 作者: Danielson, Steven R;Held, Jason M;Oo, May;Riley, Rebeccah;Gibson, Bradford W;Andersen, Julie K
• 通讯作者: Andersen, Julie K

Mass spectrometric identification of novel lysine acetylation sites in huntingtin.

亨廷顿蛋白中新的赖氨酸乙酰化位点的质谱鉴定。

• 发表时间: 2011-10
• 作者: Cong, Xin;Held, Jason M;DeGiacomo, Francesco;Bonner, Akilah;Chen, Jan Marie;Schilling, Birgit;Czerwieniec, Gregg A;Gibson, Bradford W;Ellerby, Lisa M
• 通讯作者: Ellerby, Lisa M

Confident identification of 3-nitrotyrosine modifications in mass spectral data across multiple mass spectrometry platforms.

跨多个质谱平台可靠地鉴定质谱数据中的 3-硝基酪氨酸修饰。

• 发表时间: 2011-10-19
• 作者: Li B;Held JM;Schilling B;Danielson SR;Gibson BW
• 通讯作者: Gibson BW