Mass spectrometry and imaging technologies in Geroscience

老年科学中的质谱和成像技术

• 批准号: 7898683
• 负责人: Bradford Wayne Gibson
• 金额: $ 79.36万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7898683
• 关键词: Aging; Antibodies; Area; Bioenergetics; Biological Assay; Cells; Coupled; Development; Disease model; Drug Delivery Systems; Experimental Designs; Image; Imaging technology; Institutes; Institution; Mass Spectrum Analysis; Measurement; Microscopic; Neurons; Oxidation-Reduction; Pharmaceutical Preparations; Post-Translational Protein Processing; Principal Investigator; Protein Analysis; Protein Chemistry; Proteins; Proteomics; Radial; Research Personnel; Services; Staging; Technology; Work; age related; assay development; base; cellular imaging; fluorophore; instrument; instrumentation; ranpirnase; research study; small molecule

The 'Mass Spectrometry and Imaging Technologies in Geroscience1 P30 Core will consist of an integrated suite of mass spectrometry and microscopic imaging technologies coupled to specific protein and small molecule drug and metabolite analysis assays and bioenergetic analysis. The aims of this P30 Core are to integrate the unique instrumentation facilities at the Buck Institute in imaging and mass spectrometry to target the needs of the 10 components of the U54 in structural and functional proteomics, and for the analysis of drugs and targeted metabolites that constitute this Geroscience initiative. Specifically, the imaging and mass spectrometry technologies will encompass; (i) functional and fixed-cell imaging using protein-specific fluorophores and antibodies, (ii) functional analysis of intact neurons and other cells including bioenergetic and redox measurements, (iii) development and implementation of protein chemistries, (iv) mass spectrometry-based analysis of proteins, including their identification, quantitation, interactions and posttranslational modifications, (v) mass spectrometry imaging and profiling studies, and (vi) drug and metabolite analyses. These studies will be carried out on a range of aging and aging-related disease models that constitute the components of this U54. The P30 core will consist of two main but integrated parts: 'Functional and Structural Imaging Technologies' directed by Dr. Nicholls and 'Mass Spectrometry Technologies' directed by the principal investigator, Dr. Bradford Gibson. We propose to apply and integrate these instrument-based technologies at the very earliest stages of experimental design and work closely with the other investigators to develop, optimize, and implement these mass spectrometry and imaging technologies.

“Geroscience1 P30 核心中的质谱和成像技术”将由一个集成的 与特定蛋白质和小分子相结合的一套质谱和显微成像技术 分子药物和代谢物分析测定和生物能分析。 P30 Core 的目标是 整合巴克研究所在成像和质谱方面的独特仪器设施， 针对 U54 的 10 个组件在结构和功能蛋白质组学中的需求，以及 对构成这项老年科学计划的药物和目标代谢物进行分析。具体来说， 成像和质谱技术将包括； (i) 功能性和固定细胞成像 蛋白质特异性荧光团和抗体，(ii) 完整神经元和其他细胞的功能分析，包括 生物能和氧化还原测量，(iii) 蛋白质化学的开发和实施，(iv) 基于质谱的蛋白质分析，包括其鉴定、定量、相互作用和 翻译后修饰，(v) 质谱成像和分析研究，以及 (vi) 药物和 代谢物分析。这些研究将在一系列衰老和衰老相关疾病模型上进行 构成此 U54 的组件。 P30核心将由两个主要但集成的部分组成： Nicholls 博士指导的“功能和结构成像技术”和“质谱” 技术由首席研究员 Bradford Gibson 博士指导。我们建议申请并整合 这些基于仪器的技术在实验设计的最初阶段就与 其他研究人员开发、优化和实施这些质谱和成像 技术。