Gene Targeting Core Facility

基因打靶核心设施

基本信息

批准号：
7918100
负责人：
ROBERT A KESTERSON
金额：
$ 10.7万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7918100
关键词：
Animal Experimentation Animal Model Animals Biological Models Breeding Cancer Center Clinical Nutrition Consultations Core Facility Cryopreservation DNA Disease ES Cell Line Embryo Functional disorder Gene Targeting Genes Genetic Genetically Engineered Mouse Genomics Goals Human Human Resources Image Immune system Individual Injection of therapeutic agent Intervention Knockout Mice Laboratories Link Microinjections Mission Modification Molecular Mus Nutritional Study Pathology Phenotype Postdoctoral Fellow Production Productivity Reagent Research Research Personnel Resources Rheumatism Services Stem Cell Development Students System Technology Tissues Training Transgenes Transgenic Animals Transgenic Mice Transgenic Mouse Facility Transgenic Organisms University of Alabama at Birmingham Cancer Center animal facility blastocyst cost cost effective design embryonic stem cell genetic manipulation human disease interest lectures mouse genome mouse model recombinase reconstitution vector

项目摘要

The Gene Targeting Core Facility (GTCF) is a fully established core resource to provide expert services for gene targeting of embryonic stem (ES) cells for the development of mouse models. The primary service orovided by this core is that of taking an investigator's gene targeting vector and producing ES cell lines that can be developed into chimeric mice that transmit the pertinent genomic modification(s) through their germline. Investigators utilizing this core have successfully produced a variety of gene knockouts and mouse models of disease. The GTCF is an integral component of the UAB Transgenic Mouse Facility that collectively provides expert services for not only gene targeting via the GTCF, but also blastocyst micro-injection of targeted ES cell clones or DNA constructs for the production of transgenic mouse models, as well as a host of services related to research with mice (e.g. cryopreservation of embryos, rederivation, etc.). The Transgenic Animal Facility is strategically linked with other core resources at UAB including the CCC Small Animal Imaging Core, Clinical Nutrition Research Center Small Animal Phenotyping Core, Research Animal Pathology Laboratory for phenotype assessment, and a specialized mouse-breeding core (Genetically Engineered Mouse and Marker-Assisted Genetics Core). Overall, we have premier resources to make and characterize mouse models for the Center investigators.

基因靶向核心设施（GTCF）是一个完全建立的核心资源，可为胚胎干细胞的基因靶向小鼠模型的开发。主要服务通过这种核心，将研究者的基因靶向向量并产生ES细胞系，而该基因的基因可以发展为将相关基因组修饰通过其种系传输的嵌合小鼠。利用该核心的研究人员成功产生了各种基因敲除和小鼠疾病模型。 GTCF是UAB转基因小鼠设施的组成部分，共同提供专家服务，不仅可以通过GTCF进行基因靶向，还为胚泡靶向靶向对靶向ES细胞克隆或DNA构建体的微注射，用于生产转基因小鼠模型，以及与小鼠研究有关的许多服务（例如，胚胎的冷冻保存，重新启动， ETC。）。转基因动物设施从战略上与UAB的其他核心资源联系在一起 CCC小动物成像核心，临床营养研究中心小动物表型核心，研究动物病理学实验室用于表型评估和专门的小鼠繁殖核心（基因工程的小鼠和标记辅助遗传学核心）。总体而言，我们有主要资源为中心研究人员制作和表征鼠标模型。