2D-HPLC-Tandem Mass Spectrometer for Proteomics

用于蛋白质组学的 2D-HPLC-串联质谱仪

• 批准号: 6876925
• 负责人: RICHARD B VAN BREEMEN
• 金额: $ 38.3万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876925
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; high performance liquid chromatography; liquid chromatography mass spectrometry; proteomics

DESCRIPTION (provided by applicant): A 2-dimensional (2D) microcapillary HPLC-tandem mass spectrometer (2D mu LC-MS/MS) will be purchased and used to support the research of a multidisciplinary group of 10 major NIH-funded projects (including several multi-investigator program projects) and 5 minor users. Approximately 80% of the instrument time will be utilized by NIH-funded major users, and the remaining 20% of the time will be used by the minor users and new users from on campus or in the Chicago area. This instrument will complement the MALDI TOF mass spectrometer that is already devoted to proteomics research within the Research Resources Center (RRC) of the University of Illinois at Chicago and will provide unique microcapillary HPLC capabilities and on-line solution phase protein characterization and identification. In particular this instrument will be used by many NIH-funded investigators for proteomic profiling using techniques such as isotope-coded affinity tags (ICAT). Presently, our investigators must seek collaborators at other institutions for such studies, since there are no comparable proteomics instruments available on campus. The proposed instrument will be located on the medical campus of the University of Illinois at Chicago in recently renovated space under the supervision of the P.I. Access to the instrument by new users on and off campus will be assured through the administrative oversight of an advisory committee appointed by the RRC which is a centralized campus instrumentation facility that has been in operation for over 50 years. The RRC recently hired a Ph.D. level senior mass spectrometrist to oversee the MALDI TOP mass spectrometer, and a new proteomics mass spectrometrist will be recruited to operate and maintain the proposed 2D LC-MS/MS system and carry out ICAT and similar analyses. These positions are committed for at least the useful lifetimes of these proteomics instruments. Furthermore, the Mass Spectrometry Laboratory is under the oversight of the P.I. Dr. Richard B. van Breemen, who has more than 20 years of experience in biomedical mass spectrometry. These on-going personnel commitments represent substantial support by the RRC and the university. User fees (determined by the advisory committee) will be collected to defray the costs of consumable supplies and repairs. Overall, the proposed 2D mu LC-MS/MS instrument will complement the existing proteomics resources on campus and will enhance the current research of NIH-funded users by providing analytical capabilities currently unavailable on campus.

描述（由申请人提供）：将购买一台二维（2D）微毛细管 HPLC-串联质谱仪（2D mu LC-MS/MS）并用于支持由 10 个 NIH 资助项目组成的多学科小组的研究（包括几个多研究者计划项目）和 5 个次要用户。大约80%的仪器时间将由NIH资助的主要用户使用，剩余20%的时间将由次要用户和来自校园或芝加哥地区的新用户使用。该仪器将补充伊利诺伊大学芝加哥分校研究资源中心 (RRC) 已致力于蛋白质组学研究的 MALDI TOF 质谱仪，并将提供独特的微毛细管 HPLC 功能以及在线溶液相蛋白质表征和鉴定。特别是，该仪器将被许多 NIH 资助的研究人员使用同位素编码亲和标签 (ICAT) 等技术进行蛋白质组分析。目前，我们的研究人员必须在其他机构寻找合作者进行此类研究，因为校园内没有类似的蛋白质组学仪器。拟议的仪器将位于伊利诺伊大学芝加哥分校医学院最近翻新的空间内，由 P.I. 负责监督。 RRC 是一个集中式校园仪器设施，已运行 50 多年，将通过 RRC 任命的咨询委员会的行政监督，确保校内外新用户对仪器的使用。 RRC 最近聘请了一位博士。高级质谱师负责监督 MALDI TOP 质谱仪，并将招募一名新的蛋白质组学质谱师来操作和维护拟议的 2D LC-MS/MS 系统并进行 ICAT 和类似分析。这些职位至少在这些蛋白质组学仪器的使用寿命内是承诺的。此外，质谱实验室受到 P.I. 的监督。 Richard B. van Breemen 博士在生物医学质谱领域拥有 20 多年的经验。这些持续的人员承诺代表了 RRC 和大学的大力支持。将收取用户费用（由咨询委员会确定）以支付消耗品和维修费用。总体而言，拟议的 2D mu LC-MS/MS 仪器将补充校园内现有的蛋白质组学资源，并将通过提供校园目前无法提供的分析功能来增强 NIH 资助的用户当前的研究。