Thermo Vantage triple quadrupole with FAIMS and Accela UPLC

配备 FAIMS 和 Accela UPLC 的 Thermo Vantage 三重四极杆

• 批准号: 7795994
• 负责人: KYM Francis FAULL
• 金额: $ 49.45万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-10 至 2011-07-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795994
• 关键词: 17 year old; Age; Aging-Related Process; Alzheimer' s Disease; Arts; Blood capillaries; Brain Injuries; Communication; Complex Mixtures; Computers; Detection; Development; Disease; Doctor of Philosophy; Equipment; Frequencies; Funding; Funding Applicant; Gas Chromatography; Health; High Pressure Liquid Chromatography; Homeostasis; Hormonal; Human; Immune response; Inflammation; Institutes; Ions; Laboratories; Liquid substance; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Mental disorders; Metabolic Diseases; Molecular; Molecular Weight; Parkinson Disease; Personal Satisfaction; Population; Process; Research; Research Infrastructure; Research Project Grants; Resolution; Signal Transduction; Societies; Spectrometry; System; Technology; Time; United States National Institutes of Health; Viral; Work; age related; base; capillary; flexibility; instrument; ion mobility; liquid chromatography mass spectrometry; mass spectrometer; medical schools; metabolomics; nervous system disorder; neuropsychiatry; operation; professor; public health relevance; ultra high pressure

DESCRIPTION (provided by applicant): Funds are requested for a state-of-the-art triple quadrupole mass spectrometer (QqQ) and two essential ancillary attachments - an ultra-high pressure liquid chromatography (UPLC) system, and a High- Field Assymetric Waveform Ion Mobility Spectrometry (FAIMS) attachment. This equipment will be located in the Pasarow Mass Spectrometry Laboratory (PMSL) within the Semel (formerly Neuropsychiatric) Institute at UCLA, a campus-wide core mass spectrometry laboratory. The QqQ mass spectrometers currently in the PMSL are about 17 years old and these are obsolete by any criteria. In the intervening period there have been significant improvements in sensitivity, resolution, accuracy of m/z assignment, computer control, experimental flexibility, and detection dynamic range of these instruments. UPLC brings liquid chromatographic resolution close to that attainable with capillary gas chromatography, and is an important development for high sensitivity work, high throughput work, and for work with complex mixtures. There is no UPLC system on the UCLA campus. The FAIMS technology is a new development that for some compounds lowers the background from isobaric ions, and as such can provide a significant advantage for low molecular weight analyses of complex mixtures by LC/MS, as is intended for the new instrument. The research projects for which the equipment will be used involve quantitative and qualitative analyses of a diverse range of molecules, many but not all with relatively low molecular weights (generally less that 1500 Da), in a variety of milieu. Collectively these compounds are implicated in diverse phenomena including signal transduction, cellular communication, hormonal homeostasis, addictive processes, ageing, logical thought processing, immune responses and inflammation. The application includes descriptions of eighteen collaborating laboratories with mass spectrometric analysis essential to their research projects. These groups are expected to occupy approximately 90% of instrument time. The remaining instrument time will be used by other collaborating groups as needed. Funding support is in place for all the collaborating laboratories. The PMSL has the necessary infrastructure to maintain and support the new equipment. Professor Faull, the PI on this application and the Director of the PMSL, and Postdoctoral Research Fellow Christopher Ryan, PhD, will be responsible for the day-to-day operation and maintenance of the new equipment. The PI has a strong track- record for the effective use of equipment purchased through the NIH SIG mechanism. This application represents a critical component of an effort by the UCLA Semel Institute and School of Medicine to re-place obsolete equipment within the campus core laboratories with state-of-the-art instruments for cutting edge research in metabolomics and related fields. PUBLIC HEALTH RELEVANCE: The research for which the new equipment will be used is directly relevant to many aspects of human health and disease, including cancer, metabolic diseases, brain injury, viral infectivity, psychiatric diseases, and age-related neurological diseases including Alzheimer's and Parkinson's diseases. The frequency with which many of these diseases occur in Western society is rising as the population ages. Research equipment such as that requested is crucial for understanding the molecular basis of these diseases and continued progress on these important problems that significantly impact human health and well-being.

描述（由申请人提供）：需要资金购买最先进的三重四极杆质谱仪（QqQ）和两个重要的辅助附件 - 超高压液相色谱（UPLC）系统和高场不对称波形离子淌度谱 (FAIMS) 附件。该设备将位于加州大学洛杉矶分校塞梅尔（以前的神经精神病学）研究所内的帕萨罗质谱实验室（PMSL），这是一个全校范围的核心质谱实验室。目前 PMSL 中的 QqQ 质谱仪已有大约 17 年的历史，无论从任何标准来看，它们都已经过时了。在此期间，这些仪器的灵敏度、分辨率、m/z 分配的准确性、计算机控制、实验灵活性和检测动态范围都有了显着的改进。 UPLC 使液相色谱分辨率接近毛细管气相色谱所能达到的分辨率，是高灵敏度工作、高通量工作以及复杂混合物工作的重要发展。 UCLA 校园内没有 UPLC 系统。 FAIMS 技术是一项新发展，对于某些化合物来说，可以降低同量异位离子的背景，因此可以为通过 LC/MS 进行复杂混合物的低分子量分析提供显着优势，正如新仪器的预期那样。使用该设备的研究项目涉及在各种环境中对各种分子进行定量和定性分析，其中许多但并非全部具有相对较低的分子量（通常小于 1500 Da）。总的来说，这些化合物与多种现象有关，包括信号转导、细胞通讯、激素稳态、成瘾过程、衰老、逻辑思维处理、免疫反应和炎症。该应用程序包括十八个合作实验室的描述，这些实验室的质谱分析对其研究项目至关重要。这些组预计占据大约 90% 的仪器时间。剩余的仪器时间将由其他合作小组根据需要使用。所有合作实验室均获得资金支持。 PMSL 拥有维护和支持新设备所需的基础设施。该应用的首席研究员、PMSL 主任 Faull 教授和博士后研究员 Christopher Ryan 博士将负责新设备的日常操作和维护。 PI 在有效使用通过 NIH SIG 机制购买的设备方面拥有良好的记录。该应用程序是加州大学洛杉矶分校塞梅尔研究所和医学院努力的一个重要组成部分，旨在用最先进的仪器替换校园核心实验室内的过时设备，以进行代谢组学和相关领域的前沿研究。 公共健康相关性：将使用新设备的研究与人类健康和疾病的许多方面直接相关，包括癌症、代谢疾病、脑损伤、病毒感染、精神疾病以及与年龄相关的神经系统疾病，包括阿尔茨海默氏症和老年痴呆症。帕金森病。随着人口老龄化，西方社会许多此类疾病的发生频率正在上升。所需的研究设备对于了解这些疾病的分子基础以及在这些严重影响人类健康和福祉的重要问题上持续取得进展至关重要。

项目成果

Lipidomic profiling of sinus mucosa from patients with chronic rhinosinusitis.

慢性鼻窦炎患者鼻窦粘膜的脂质组学分析。

• 发表时间: 2015-04
• 作者: Fazlollahi, Farbod;Kongmanas, Kessiri;Tanphaichitr, Nongnuj;Mallen;Gopen, Quinton;Faull, Kym F;Suh, Jeffrey D
• 通讯作者: Suh, Jeffrey D