STP & TIP ARE ESSENTIAL FOR HERPESVIRUS SAIMIRI ONCOGENICITY

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• 批准号: 6591296
• 负责人: Samuel Monroe DUBOISE
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6591296
• 关键词: Mammalia; animal tissue; communicable diseases; virus

Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets Parental "VS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such growth transformation Wild-type "VS produced fatal lymphoma within 19 to 20 days of experimental infection of common marmosets, while "VS ASTP-C488 and "VS ATip were nononcogenic Virus was repeatedly isolated from the peripheral blood of marmosets infected with mutant virus for more than 5 months These results demonstrate that STP-C488 and Tip are not required for replication or persistence, but each is essential for transformation in cell culture and for lymphoma induction in common marmosets PUBLICATIONS Duboise, SM, Guo, J, Czajak, S, Desrosiers, RC and Jung, JU STP and Tip are Essential for Herpesvirus Saimiri Oncogenicity J Virol 1998; 72:1308-1313

疱疹病毒Saimiri（HVS）亚组菌株488的突变形式 在STP-C488或尖端中删除了删除 在细胞培养中测试了HVS突变体的转换电位 在常见的果果中，父母“与亚组C菌株488 永生的普通果果果t淋巴细胞在体外 白介素-2独立的生长，但两个缺失突变体都没有 产生了这种生长转化野生型“与致命产生的致命 常见实验感染的19至20天内的淋巴瘤 马莫果，而“ vs vs astp-c488和”与ATIP是非结构病毒 反复从果泥的外周血中分离出来 被突变病毒感染超过5个月 证明复制不需要STP-C488和TIP 持久性，但每个都对细胞培养的转化至关重要 以及用于淋巴瘤的淋巴瘤诱因， SM，Guo，J，Czajak，S，Desrosiers，RC和Jung，Ju Stp和Tip是 Saimiri致癌性J Virol 1998年至关重要； 72：1308-1313