ROLE FOR HERPESVIRUS SAIMIRI ORF14 IN TRANSFORMATION & PERSISTENT INFECTION

疱疹病毒 SAIMIRI ORF14 在转化中的作用

• 批准号: 6453737
• 负责人: Samuel Monroe DUBOISE
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6453737
• 关键词: Mammalia; communicable diseases; skeletal system; virus

The product of open reading frame 14 (orft4) of herpesvirus saimiri (HVS) exhibits significant homology with mouse mammary tumor virus superantigen orfl4 encodes a 50-kDa secreted glycoprotein, as shown previously (Z Yao, E Maraskovsky, M K Spriggs, J I Cohen, R J Armitage, and M R Alderson, J Immunol 156:3260-3266, 1996) orfl4 expressed from recombinant baculovirus powerfully induces proliferation of CD4-positive cells originating from several different species To study the role of orfl4 in transformation, a mutant form of HVS (HVS orfl4) was constructed with a deletion in the orfl4 gene The transforming potential of HVS orfl4 was tested in cell culture and in common marmosets Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, while the HVS orft4 mutant did not produce such a growth transformation In addition, HVS orfl4 was nononcogenic in common marmosets In contrast to oth er nononcogenic HVS mutant viruses which were repeatedly isolated from peripheral blood mononuclear cells of infected marmosets for more than 5 months, HVS orfl4 did not persist at a high level in vivo These results demonstrate that orfl4 of HVS is not required for replication but is required for transformation and for high-level persistence in vivo PUBLICATIONS Duboise, SM, Guo, J, Czajak, S, Lee, H, Veazey, Desrosiers, RC and Jung, JU A role for herpesvirus saimiri orf14 in transformation and persistent infection J Virol 1998; 72:6770-6776

疱疹病毒的开放阅读框架14（ORFT4）的产物 Saimiri（HVS）与小鼠乳腺肿瘤具有显着同源性 病毒超抗原ORFL4编码50 kDa的分泌糖蛋白，为 先前显示（Z Yao，E Maraskovsky，M K Spriggs，J I Cohen，R J Armitage和M R Alderson，J Immunol 156：3260-3266，1996）ORFL4 通过重组杆状病毒表达有力诱导 CD4阳性细胞的增殖，源自几种不同 物种研究ORFL4在转化中的作用，一种突变形式 HVS（HVS ORFL4）在ORFL4基因中构建 在细胞培养和 普通果果族父母HVS亚组C菌株488永生 常见的摩尔果T淋巴细胞在体外与白细胞介素-2独立的 增长，而HVS ORFT4突变体没有产生这种增长 此外，转化，HVS ORFL4在常见 马莫果与非结构性HVS突变病毒相比 从外周血单核细胞中反复分离 HVS ORFL4感染了5个月以上，不持续 在体内高水平的这些结果表明，HV的ORFL4是 复制不需要，而是转换和 为了在体内出版物Duboise，SM，Guo，J，J， Czajak，S，Lee，H，Veazey，Desrosiers，RC和Jung，JU 疱疹病毒saimiri orf14在转化和持续感染中 Virol 1998; 72：6770-6776