CRYO CRYSTALLOGRAPHY OF PHOTOCYCLE INTERMEDIATE OF PHOTOACTIVE YELLOW PROTEIN

光活性黄蛋白光循环中间体的冷冻晶体学

• 批准号: 6586651
• 负责人: ULRICH K GENICK
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586651
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Photoactive Yellow Protein (PYP) is a small, cytosolic photoreceptor of the bacterium Ectothiorhodaspira halophila. Its small size and its ability to undergo the photocycle even in a crystal make it ideal to study structural changes in proteins by time-resolved crystallography. The determination of the structure of the early intermediate would complete the structural determination of all known structural intermediates of PYP.

光活性黄色蛋白 (PYP) 是一种小型细胞质 嗜盐外硫菌螺旋体细菌的光感受器。 它是 尺寸小，即使在晶体中也能进行光循环 使其成为通过时间分辨研究蛋白质结构变化的理想选择 晶体学。 早期结构的确定 中间体将完成所有已知的结构测定 PYP的结构中间体。