SAXS STUDY OF THE LIGHT SENSING REACTION OF PHYTOCHROME

PHYTOCHROME 光传感反应的 SAXS 研究

• 批准号: 7370435
• 负责人: ULRICH K GENICK
• 金额: $ 0.53万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370435
• 关键词: SAXS; STUDY; LIGHT; SENSING; REACTION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phytochromes are photoreceptors found in plants and certain bacteria where they sense the duration and intensity of red and far-red light. According to the received light signals, phytochromes then control or modulate virtually every aspect of plant metabolism and development. Preliminary biochemical experiments suggest that light activation of phytochromes causes large inter-domain motions which are essential for signal transduction. To investigate the structural basis of this important signaling reaction, we will extend earlier static SAXS experiments by Tokutomi, et al. (FEBS 274, pp. 139-42 (1989). Using the higher resolution data obtainable at the superior SAXS facility at SSRL, we will be able to generate a more detailed, higher fidelity model of phytochrome. Additionally, we will investigate the role of domain rearrangements in the light-activated signaling reaction of phytochrome using time-resolved SAXS

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。光敏色素是在植物和某些细菌中发现的光感受器，它们感知红光和远红光的持续时间和强度。根据接收到的光信号，光敏色素随后控制或调节植物代谢和发育的几乎各个方面。初步生化实验表明，光敏色素的光激活会引起大的域间运动，这对于信号转导至关重要。为了研究这一重要信号反应的结构基础，我们将扩展 Tokutomi 等人早期的静态 SAXS 实验。 （FEBS 274，第 139-42 页（1989）。使用 SSRL 高级 SAXS 设施获得的更高分辨率数据，我们将能够生成更详细、更高保真度的光敏色素模型。此外，我们将研究光敏色素的作用使用时间分辨 SAXS 分析光敏色素光激活信号反应中的结构域重排