A SAXS STUDY OF THE LIGHT-SENSING REACTION OF PLANT AND BACTERIAL PHYTOCHROMES

植物和细菌光敏色素光敏反应的 SAXS 研究

• 批准号: 7598127
• 负责人: ULRICH K GENICK
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598127
• 关键词: Bacteria; Biological; Biological Process; Computer Retrieval of Information on Scientific Projects Database; Data; Family; Funding; Goals; Grant; Growth; Homology Modeling; Individual; Institution; Length; Light; Modeling; Molecular; Organism; Photoreceptors; Phytochrome; Plants; Process; Proteins; Reaction; Research; Research Personnel; Resources; Roentgen Rays; Source; Sunlight; Time; United States National Institutes of Health; Water; day; light intensity; molecular modeling; protein folding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Protein photoreceptors allow biological organisms to sense, process and transmit the information contained in ambient light. Are light intensities sufficient to support growth? What time of day / year is it? Is a neighboring organism blocking access to sunlight? Plants and bacteria use photoreceptors from the phytochrome family to find the answer to all of these questions. We want to understand the molecular mechanism underlying phytochromes¿ biological function. Phytochromes are large, water-soluble multi-domain photoreceptors. Light activation of phytochromes has been shown to induce large-scale conformational changes. The protein fold for most phytochrome domains is known, thus allowing us to built molecular model of these domains by homology modeling. The goal of the proposed research is to use Small Angle X-ray scattering data to assemble the molecular models of individual domains into a model for the full-length phytochrome. In addition we would want to determine how this domain arrangement changes as a result of light-activation.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 蛋白质光感受器允许生物有机体感知、处理和传输环境光中包含的信息，光强度是否足以支持生长？植物和细菌是否使用光感受器来获取阳光？我们希望了解光敏色素家族的分子机制，以找到所有这些问题的答案。光敏色素是大型的水溶性多结构域光感受器，光敏色素的光激活已被证明可引起大规模的构象变化，因此我们可以建立这些结构域的分子模型。该研究的目标是使用小角度 X 射线散射数据将各个域的分子模型组装成全长光敏色素的模型。确定该结构域如何因光激活而改变排列。