A SAXS STUDY OF THE LIGHT-SENSING REACTION OF PLANT AND BACTERIAL PHYTOCHROMES

植物和细菌光敏色素光敏反应的 SAXS 研究

• 批准号: 7721898
• 负责人: ULRICH K GENICK
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721898
• 关键词: Bacteria; Biological; Biological Process; Computer Retrieval of Information on Scientific Projects Database; Data; Family; Funding; Goals; Grant; Growth; Homology Modeling; Individual; Institution; Length; Light; Modeling; Molecular; Organism; Photoreceptors; Phytochrome; Plants; Process; Proteins; Reaction; Research; Research Personnel; Resources; Roentgen Rays; Source; Sunlight; Time; United States National Institutes of Health; Water; day; light intensity; molecular modeling; protein folding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Protein photoreceptors allow biological organisms to sense, process and transmit the information contained in ambient light. Are light intensities sufficient to support growth? What time of day / year is it? Is a neighboring organism blocking access to sunlight? Plants and bacteria use photoreceptors from the phytochrome family to find the answer to all of these questions. We want to understand the molecular mechanism underlying phytochromes? biological function. Phytochromes are large, water-soluble multi-domain photoreceptors. Light activation of phytochromes has been shown to induce large-scale conformational changes. The protein fold for most phytochrome domains is known, thus allowing us to built molecular model of these domains by homology modeling. The goal of the proposed research is to use Small Angle X-ray scattering data to assemble the molecular models of individual domains into a model for the full-length phytochrome. In addition we would want to determine how this domain arrangement changes as a result of light-activation.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 蛋白质感受器使生物生物可以感知，处理和传输环境光中所包含的信息。 光强度足以支持增长吗？一天中 /年什么时间？邻近的生物会阻止进入阳光吗？植物和细菌使用植物色素家族的感光体来找到所有这些问题的答案。 我们想了解植物色素的分子机制吗？生物功能。 植物色素是大的水溶性多域光感受器。植物色素的光激活已被证明会诱导大规模构象变化。已知大多数植物色素结构域的蛋白质折叠，从而使我们能够通过同源性建模来建立这些域的分子模型。拟议的研究的目的是使用小角度X射线散射数据将单个结构域的分子模型组装成全长植物色素的模型。 此外，我们要确定由于光激活而导致该域的排列如何变化。